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Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora as well as preparation method and application thereof

A fermented liquid and angelica technology, applied in the field of angelica fermented liquid and its preparation, can solve the problems of long decoction time, slow treatment process, difficult dissolution, etc., and achieve the effects of increasing content, removing free radicals, and alleviating aging

Active Publication Date: 2021-10-08
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the field of traditional Chinese medicine, it has a good curative effect on the treatment of chronic diseases, but it has been criticized for its slow treatment process, bitter taste, and long cooking time.
Moreover, most of the active ingredients of traditional Chinese medicine are wrapped in dense cell walls, which are difficult to dissolve

Method used

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  • Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora as well as preparation method and application thereof
  • Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora as well as preparation method and application thereof
  • Angelica sinensis fermentation liquor with effects of resisting oxidation, resisting aging and regulating intestinal flora as well as preparation method and application thereof

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preparation example Construction

[0038] The invention provides a kind of preparation method of angelica fermented liquid, comprises the following steps:

[0039] (1) After the angelica is soaked in water, decoct, filter and collect the filtrate, add water to the filter residue, continue to decoct, filter and collect the filtrate, combine the two filtrates, concentrate and centrifuge to obtain the angelica water extract;

[0040] (2) fermented angelica water extract:

[0041] Lactobacillus plantarum LZU-J-TSL6 is mixed with LZU-S-ZCJ, inoculated in water extract of Angelica sinensis and fermented to obtain Angelica sinensis fermented liquid.

[0042] In the step (1) of the present invention, the soaking time is preferably 8-16 hours, more preferably 10-14 hours, even more preferably 12 hours.

[0043] In the step (1) of the present invention, the volume ratio of decocting Angelica sinensis and water for the first time is preferably 1:8~12, more preferably 1:8~10, even more preferably 1:10, the decoction time ...

Embodiment 1

[0052] Add 10 times the volume of Angelica sinensis water, soak for 12 hours, decoct for 1 hour, collect the filtrate by filtration, add 8 times the volume of water to the filter residue, continue to decoct for 1 hour, collect the filtrate by filtration, combine the two filtrates, and concentrate the filtrate juice at 0.6g / mL, 4°C , centrifuged at 3000rmp, took the supernatant, and set aside. Mix Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ at a volume ratio of 1:3, and the concentration of the two strains is 5×10 12 CFU / mL, with 4% inoculum size, inoculated in the above-mentioned Angelica water extract prepared with 1 mL of extract per 0.6 g of Angelica sinensis. Under the condition of a fermentation temperature of 36° C., ferment for 72 hours to obtain Angelica fermented liquid.

Embodiment 2

[0054] Add 8 times the volume of Angelica sinensis water, soak for 8 hours, decoct for 0.5 hours, filter and collect the filtrate, add 6 times the volume of water to the filter residue, continue to decoct for 0.5 hours, filter and collect the filtrate, combine the two filtrates, and concentrate the filtrate to 0.4g / mL, Centrifuge at 3000rmp at 4°C, take the supernatant, and set aside. Mix Lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ at a volume ratio of 3:1, and the concentration of the two strains is 1×10 12 CFU / mL, inoculate with 3% inoculum amount in the above-mentioned angelica aqueous extract prepared with 1 mL extract per 0.4 g of angelica. Under the condition of a fermentation temperature of 37°C, ferment for 100 hours to obtain Angelica fermented liquid.

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Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and provides angelica sinensis fermentation liquor with the effects of resisting oxidation, resisting aging and regulating intestinal flora as well as a preparation method and application thereof. The preparation method comprises the following steps: soaking angelica sinensis in water, then decocting, filtering to collect the filtrate, adding water into filter residues, continuing decoction, filtering to collect the filtrate, combining the two filtrates, conducting concentration and centrifugation, and obtaining an angelica sinensis water extract; and fermenting the angelica sinensis water extract: mixing lactobacillus plantarum LZU-J-TSL6 and LZU-S-ZCJ, inoculating the mixture into the angelica sinensis water extract, and fermenting to obtain the angelica sinensis fermentation liquor. The angelica sinensis fermentation liquor obtained by the preparation method has the effects of resisting oxidation and aging, well scavenging free radicals and regulating the intestinal flora, and has a good health-care effect.

Description

technical field [0001] The invention relates to the technical field of fermentation of traditional Chinese medicines, in particular to an angelica fermented liquid with the functions of anti-oxidation, anti-aging and regulation of intestinal flora, as well as its preparation method and application. Background technique [0002] Aerobic organisms in the world will gradually be exposed to the environment produced by oxidants or electrophiles, which is affected by the process of enzyme reactions in the body and external harmful substances. These oxidative poisons will have serious effects on the body. In order to avoid these bad effects, the body has evolved a complex but stable antioxidant defense system to maintain the redox reaction in the body. ROS (reactive oxygen species) produced excessively by cells in the body and ROS produced by electrophiles can induce free radical chain reactions, cross-link and polymerize biomacromolecules in cells, and generate malondialdehyde (MD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A61K36/232A61P39/06A61P1/00C12R1/25
CPCC12P1/04A61K36/232A61P39/06A61P1/00A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/19
Inventor 张春江卢雪蕊李君翔
Owner LANZHOU UNIVERSITY
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