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Nucleic acid aptamer specifically bound with human FXYD2 gamma a and derivative, screening method and application thereof

A nucleic acid aptamer and screening method technology, applied in biochemical equipment and methods, library screening, biological material analysis, etc., can solve the problems of unstable and irreversible denaturation of antibodies, large batch-to-batch differences, long production time, etc., to achieve Good application prospects, short cycle, easy to save the effect

Active Publication Date: 2021-10-08
SHENZHEN PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The existing FXYD2γa detection method is based on the detection method of antibody or its derivatives, but the antibody is unstable, prone to irreversible denaturation, large batch-to-batch variation, long production time and high cost. The prior art has not reported the nucleic acid adaptation of FXYD2γa. Ligand Screening Studies

Method used

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  • Nucleic acid aptamer specifically bound with human FXYD2 gamma a and derivative, screening method and application thereof
  • Nucleic acid aptamer specifically bound with human FXYD2 gamma a and derivative, screening method and application thereof
  • Nucleic acid aptamer specifically bound with human FXYD2 gamma a and derivative, screening method and application thereof

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Embodiment

[0034] The nucleic acid aptamer directed at FXYD2γa is the development object of this paper, and is finally the protection object of this paper. The scope of this paper relates to the obtained nucleic acid aptamer specifically binding to FXYD2γa, substances (such as : probes, detection reagents, kits, sensors, etc.), applications (such as diagnostic applications, preparation applications, etc.), however, those skilled in the art should understand that the protected objects herein are not limited to these examples.

[0035]The present invention provides a nucleic acid aptamer that specifically binds to human FXYD2γa and can be used to detect pancreatic islet β cells. The nucleotide sequence of the nucleic acid aptamer is: 5'-AACGAAGGTATACAAAAATATATATAAATATCTACTACG-3' (SEQ ID NO.1).

[0036] Optionally, any base on the nucleotide sequence of the above-mentioned nucleic acid aptamer can be phosphorylated, methylated, aminated, sulfhydrylated or isotoped. Biotin, digoxigenin, fluo...

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Abstract

The invention provides a nucleic acid aptamer specifically bound with human FXYD2 gamma a and a derivative, a screening method and application thereof. The nucleotide sequence of the nucleic acid aptamer obtained through the screening method is 5'-AACGAAGGTATACAAAAATATAATATAAATATCTACTACG-3', and compared with an FXYD2 gamma a specific antibody, the nucleic acid aptamer has the advantages of being short in screening period, low in cost, stable, easy to store, capable of being transported at normal temperature, wide in application and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a nucleic acid aptamer specifically binding to human FXYD2γa and its derivatives, a screening method and application. Background technique [0002] Nucleic acid aptamer (Aptamer) is a structured oligonucleotide sequence (RNA or DNA ), nucleic acid aptamers have similar functions to antibodies, but have more advantages than antibodies, with higher affinity and specificity; no immunogenicity; can be chemically synthesized, low cost; can be labeled; qualitative, Easy to save and other advantages. The target molecules of nucleic acid aptamers are more extensive, including metal ions, amino acids, nucleic acids, polypeptides, proteins, and expanded from single targets to complex targets such as complete virus particles and cells. Therefore, nucleic acid aptamers have broad application prospects. [0003] Diabetes mellitus is a metabolic disease characterized by hyperglycemia due to def...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N33/573C40B30/04C40B50/06
CPCC12N15/115G01N33/573C40B30/04C40B50/06C12N2310/16C12N2310/315C12N2310/3181G01N2333/914G01N2800/042G01N2800/52G01N2800/50
Inventor 贾黎静罗茜陈南迪郑沛林
Owner SHENZHEN PEOPLES HOSPITAL
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