Application of soybean gene promoters pEIF1 and pEIF1-I in soybean, arabidopsis thaliana and tobacco

Abstract

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of soybean gene promoters pEIF1 and pEIF1-I in soybean, arabidopsis thaliana and tobacco. According to the invention, a gene RPS28 and a gene EIF1 are cloned from soybeans; a promoter pEIF1 and a promoter pEIF1-I are obtained through a CTAB method, then a soybean stable transformation vector is constructed, expression of pEIF1 and pEIF1-I driven GUS protein in all stages of soybean genetic transformation is achieved, it is found that pEIF1 and pEIF1-I driven GUS is expressed in cotyledons, radicles, germs, true leaves, compound leaves, buds, petioles, internodes, roots and root nodules, it is indicated that pEIF1 and pEIF1-I are pan-expression promoters, and the activity of the expression product of the GUS protein in the soybean is measured. In addition, the transformation of pEIF1 and pEIF1-I driven GUS protein in transformation of arabidopsis thaliana and tobacco is also realized.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, in particular to a soybean gene promoter pEIF1 and pEIF1-I Application in soybean, Arabidopsis and tobacco. Background technique [0002] Promoters are an important part of regulating gene expression in plants. According to the source, promoters can be divided into exogenous promoters and endogenous promoters. Exogenous promoters can regulate gene expression heterologously, but transgenic safety issues often arise when preparing transgenic materials, especially commonly used promoters derived from viruses, such as the tobacco mosaic virus 35S promoter. In addition, there is the potential for transgene silencing to occur when the same promoter is used in the same transgenic event. Therefore, the endogenous promoter is a more favored promoter for the preparation of transgenic materials. According to the expression characteristics, promoters are divided into constitutive prom...

AI Technical Summary

Problems solved by technology

In addition, in terms of screening methods, the screening conditions of the reported constitutive promoters are mostly incomplete. Most of the promoters are obtained from the tissue expression in a single period of soybean growth and development, while the constitutive expression in soybean root nodule development The promoter of the

In terms of screening technology, most of the reported promoters are detected by the traditional qRT-PCR method, and there are not many reports on the detection of expression levels by high-throughput RNA sequencing methods

Literature (Ning Zhang, Leah K. McHale, John J. Finer. Isolation and characterization of “GmScream” promoters that regulate highly expressing soybean (Glycine maxMerr.) genes[J]. Plant Science, 2015, 241.) According to soybean strains The RNA sequencing data of 31 libraries of Williams82 (WS82) screened 10 expression genes of possible constitutive promoters, but none of them involved a continuous process of soybean growth and development and expression in root nodule development

At the same time, when multiple genes are transferred in the same plant, it is easy to cause gene silencing if driven by the same promoter

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