Applications of highland barley fermentation broth
A technology for fermentation broth and highland barley, which is applied in the directions of medical preparations containing active ingredients, cosmetic preparations, preparations for skin care, etc., to achieve the effects of stabilizing skin structure, easy control of conditions, and improving skin repairing activity
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Embodiment 1
[0034] The preparation of embodiment 1 highland barley fermented liquid
[0035] The base material of the fermentation product is highland barley, which is produced in Lhasa, Tibet, in the pan-Himalayan region. The highland barley grains containing the bran are washed with deionized water, dried, and powdered by a milling machine, and passed through a 20-100 mesh sieve to obtain the highland barley powder. Mix the highland barley powder with deionized water, and the content of the highland barley powder in the fermentation medium / culture solution is 4wt%; after high temperature and high pressure sterilization, use Saccharomyces cerevisiae for cooling to ferment under the conditions of 26~30°C and 150~250rpm 48 hours.
[0036] Preparation of barley fermented liquid:
[0037](1) Centrifuge the fermentation product at 4-10°C and 6000rpm for 10-30min, and take the supernatant;
[0038] (2) Adjust the pH to 5.0-7.0, bathe in water at 80-90°C for 30-60 minutes, centrifuge at 4000...
Embodiment 3
[0059] Example 3 Efficacy Test of Highland Barley Fermented Liquid on 3D Recombined Human Epidermis Model
[0060] The in vitro human recombinant epidermal model can simulate the growth cycle of normal human epidermis, and evaluate the safety and efficacy of the test substance through the proliferation and differentiation of epidermal keratinocytes and the structure and components of the epidermis.
[0061] Test Methods:
[0062] A 3D human recombinant epidermal model was constructed using a hanging cell culture chamber Transwell (Corning) matching a 12-well plate. When the human keratinocytes in the culture flask are expanded to 70-80% confluence, the cells are digested and harvested, the cells are resuspended with the 3D epidermis model culture medium, and inoculated into the Transwell. The 3D epidermis model was cultured in the submerged phase at the initial stage after inoculation, and the gas-liquid phase culture was started from the 4th day until the end. The experimen...
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