SSR molecular marker of fruit mulberry variety 'Yueshen 123', core primer group, kit and application thereof

Abstract

The invention discloses an SSR molecular marker of a fruit mulberry variety 'Yueshen 123', a core primer group, a kit and application thereof. According to the SSR molecular marker, the core primer group, the kit and the application thereof, a fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed by a mulberry genome sequence are screened, which confirms that core primers marked with M10360 and M29602 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 123', and the new fruit mulberry variety 'Yueshen 123' can be identified from fruit mulberry strains and can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 123'. The 'Yueshen 123' is identified by directly detecting the M10360 marker and/or the M29602 marker of the 'Yueshen 123', the uncertainty of external morphological characteristics on identification of the 'Yueshen 123' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 123', and the SSR molecular marker is beneficial to popularization, utilization and protection of the variety.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to the SSR molecular marker of a fruit mulberry variety 'Yuechen 123' and its core primer set, kit and application. Background technique [0002] Mulberry fruit is a kind of characteristic and excellent fruit resource, and it is the representative of the third-generation fruit. It has been listed as "both food and medicine" by the Ministry of Health. In recent years, with the improvement of people's living standards and health awareness The nutritional and health value of mulberry fruit is increasingly concerned by the society. As a new fruit variety, mulberry fruit is increasingly loved by people because of its unique taste, rich nutrition and high active substances. Into the families of ordinary people, accepted and loved by the citizens. In addition to fresh food, mulberries are processed and developed into mulberry juice, mul...

AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

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