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In-situ enrichment method for nematodes in soil and application of in-situ enrichment method

An in-situ enrichment and nematode technology, which is applied in animal husbandry and other fields, can solve the problems of productivity threatening the stability of the ecosystem, efficient utilization of resources, etc., and achieve obvious effects of increasing production and quality, rapid growth, and low production costs

Pending Publication Date: 2021-09-17
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil phosphorus deficiency and productivity decline caused by red soil acidification have seriously threatened the stability of the ecosystem and the efficient use of resources

Method used

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  • In-situ enrichment method for nematodes in soil and application of in-situ enrichment method
  • In-situ enrichment method for nematodes in soil and application of in-situ enrichment method
  • In-situ enrichment method for nematodes in soil and application of in-situ enrichment method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The nematodes cultivated in the present invention are Protobaculum nematodes. Such as figure 1 As shown, the biological characteristics of Protobaculum nematodes are as follows: Microbacula, Microbacula, and Microbaculidae. The body of the worm is medium in size, thin and strong, until it bends slightly to the abdomen after being killed by heat. The horny layer is finely ringed, with occasional vertical stripes. The lip area is not constricted. Mouth straight, narrow, 2-3 times the diameter of the labial area. Anterior mouth mostly ossified, posterior mouth without pharyngeal teeth or teeth. The middle of the esophagus is dilated. Females have double gonads, ovaries fold back, and vulva is located in the middle of the body. Coptus spines do not fuse, coaptum extends to the end of the tail, and the front stretches, with 8 or 9 pairs of papillae, 1 or 2 of which are located in front of the cloaca. Male lateral tail glands are posterior. The tail of the female inse...

Embodiment 2

[0033] Corn field planting experiment

[0034] 1. Test treatment: Two treatments were set up in this experiment, the control treatment (CK) of applying Protobaculum nematodes and not applying nematodes, and the area of ​​the test plot was 20m 2 (5m×4m), each treatment was repeated 3 times.

[0035] 2. Variety and planting method: The corn seed is Suyu 24, which has excellent characteristics such as high disease resistance, drought resistance and high temperature resistance. The seeding rate is generally 20kg / hm 2 , the spacing between plants and rows is 20cm×40cm, the sowing depth is 10-15cm, and the planting density is 68000 plants / hm 2 .

[0036] 3. Nutrient management: In corn planting, the application rates of urea, calcium magnesium phosphate fertilizer and potassium fertilizer are 152.72kg N / hm respectively 2、116.20kg P 2 o 5 / hm 2 and 166.32kg K 2 O / hm 2 . The pig manure is completely decomposed by dry-wet separation, composting and fermentation, etc. After de...

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Abstract

The invention discloses an in-situ enrichment method for nematodes in soil. The method comprises the following steps: (1) weighing red soil from a test plot, adding agricultural organic wastes and a microbial agent, fully and uniformly mixing the materials, and carrying out adaptive culture on the nematodes in a natural field environment to obtain the nematodes preserved in an adaptive culture medium; wherein the nematodes are bacterial-feeding nematodes; (2) backfilling the culture medium rich in the bacterial-feeding nematodes into the soil of the test plot; and (3) performing field management on the soil of the test plot, and maintaining the soil moisture to be stable. According to the method, the in-situ growth environment of the nematodes is improved, and the reproductive capacity and the yield of the protorhabditis nematodes are improved; on the basis of a biological introduction long-term positioning test of the Yingtan Red Soil Ecological Experiment Station of Chinese Academy of Sciences, the total number of the soil nematodes in red soil and the number of the protorhabditis nematodes are increased; and field trials show that the red soil alkaline, activity of acid phosphatase and the content of available phosphorus are remarkably increased by adding the protorhabditis nematodes, and the growth condition and quality of corn are improved.

Description

technical field [0001] The invention relates to the technical field related to soil animal breeding, in particular to an in-situ enrichment method and application of Protobadge nematodes in soil. Background technique [0002] Phosphorus is one of the essential nutrients for plant growth. It is not only a component of many important organic compounds in plants, but also participates in various physiological processes in plants in various ways. 74% of the cultivated land in my country is deficient in phosphorus, and more than 95% of the phosphorus in the soil is invalid phosphorus, which is difficult to be absorbed and utilized by plants. In agricultural production, highly water-soluble phosphate fertilizers are often used to meet the needs of plants for phosphorus. However, after phosphate fertilizers are applied to the soil, insoluble phosphates are easily formed, which are quickly absorbed and fixed by soil minerals or immobilized by microorganisms. The southeastern hilly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 蒋瑀霁薛敬荣栾璐李辉信郑洁孙波
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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