Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus

A technology for tomato chlorosis virus and tomato yellow leaf curl, which is applied in the field of plant protection, can solve the problems of virus inoculation accuracy limitation, high preparation difficulty, cost, etc., and can meet the requirements of resistance identification, high material survival rate, and increase in Effect of selection range

Inactive Publication Date: 2021-09-17
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In production and scientific research, there are many reports on the use of whitefly and infectious clones for virus inoculation, but the use of whitefly inoculation needs to obtain whitefly populations carrying different viruses, because whitefly simultaneously transmits tomato yellowing. For leaf curl virus and tomato chlorotic virus, it is difficult to obtain whitefly populations carrying different viruses, the inoculation conditions are not easy to control, and the accuracy of virus inoculation is limited in the study of virus-host interaction; the invasive clone of virus It can achieve timing and quantitative inoculation of viruses, but it is difficult to prepare, develop, obtain, and costly, making it difficult to apply on a large scale
In addition, there are few reports on virus grafting and inoculation techniques. Only Yu Li et al. reported that the grafting technique was used to achieve the grafting and inoculation of tomato yellow leaf curl virus. However, this method uses diseased tomato materials as rootstocks and healthy tomato materials as scion When grafting, only two true leaves of the rootstock material are kept, and the seedling age of the rootstock and the poisonous source need to be consistent. The rootstock material used as the poisonous source can only be used once. Repeated inoculation requires a large number of poisonous sources to be propagated, which consumes a lot of manpower and material resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus
  • Single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus
  • Single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A single leaf grafting and inoculation method of tomato yellow leaf curl virus and tomato chlorosis virus, the steps are as follows:

[0033] S1, get tomato seedlings or side shoots grown to 4 weeks old, select a suitable node, remove healthy leaves from the node, and make a 30 ° oblique incision on the stem of the recipient seedling;

[0034] S2, get the single leaf with good growth as the scion material, cut the petiole of the inoculated single leaf into 30 ° wedge, insert it obliquely in the otch, and fix it with grafting clips;

[0035] S3. Put the newly grafted tomato seedlings in an artificial climate chamber with controllable temperature and humidity and no whiteflies, and adjust the temperature and humidity conditions of the climate chamber for cultivation.

[0036] The conditions in the climate box are set as follows: after grafting to within 2 weeks, the temperature of the climate box is kept at 25°C throughout the day, the humidity is kept at 90%, and the lig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus, which comprises the following steps: S1, rootstock treatment: taking tomato seedlings or lateral branches, selecting proper nodes, removing healthy leaves from the nodes, and making oblique cuts on stems of receptor seedlings; S2, scion treatment: taking a single leaf with good growth vigor as a scion material, and cutting a petiole inoculated with the single leaf into a wedge shape; S3, cuttage grafting: obliquely inserting the scions into the cuts of the rootstocks, fixing the scions with grafting clamps, and culturing the scions. The single-leaf grafting inoculation method for tomato yellow leaf curl virus and tomato chlorosis virus is not affected by virus transmission mediators and is not limited by seasons, grafting materials are easy to obtain, and operation is easy and convenient.

Description

technical field [0001] The invention relates to the technical field of plant protection, in particular to a method for single-leaf grafting and inoculation of tomato yellow leaf curl virus and tomato chlorosis virus. Background technique [0002] Tomato yellow leaf curl virus (Tomato yellow leaf curl virus) and tomato chlorosis virus (TomatoChlorosis virus) are two worldwide tomato virus diseases transmitted by Bemisia tabaci. They occur and distribute widely in my country, seriously endangering tomato in my country. Production. At present, the application of chemical agents to control the virus-transmitting vector Bemisia tabaci is the main control method for this type of virus disease, but Bemisia tabaci is very easy to develop drug resistance, chemical control is likely to cause environmental and food pollution, and reduce the quality of agricultural products. Disease species is the most fundamental and effective measure. [0003] The research on the resistance of tomato...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G2/38
CPCA01G2/30A01G2/38
Inventor 张爱红杨菲邸垫平霍良占李希望陈微赵元业
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products