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A kind of rapd reaction system and kit of Euonymus peach leaf

A reaction system and technology of Euonymus peach leaf, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of lack of universality, difficulty in guaranteeing the repeatability and stability of experimental results, and few applications of Euonymus peach leaf, etc. , to achieve the effect of good basic theoretical guiding significance, high stability of results, and clear bands

Active Publication Date: 2022-07-22
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, RAPD technology has been widely used in plants such as wheat, star grass, fufangteng, pomegranate, peanut, etc., but it is rarely used in Euonymus peach leaf. At the same time, RAPD molecular markers also have some shortcomings, such as RAPD reaction does not The uniform reaction conditions and reaction system are not universal, and the RAPD reaction is easily affected by the experimental conditions, so it is difficult to guarantee the repeatability and stability of the experimental results

Method used

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  • A kind of rapd reaction system and kit of Euonymus peach leaf
  • A kind of rapd reaction system and kit of Euonymus peach leaf
  • A kind of rapd reaction system and kit of Euonymus peach leaf

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Experimental method

[0031] (1) Extraction and detection of genomic DNA

[0032] 10 fresh peach leaves from Longjing Nursery and Chaoyangchuan International Airport Nursery Base in Jilin Province were collected using the modified urea method (Zheng D H, Van K, Lee S H. Molecular diversity and relationshipsamong elite maize inbreds from US and CIMMYT populations and currentheteroticgroups in China[J].Hereditas,2010,145(4):182-193) extracted the genomic DNA of Euonymus peach leaves, and detected the quality of the genome DNA of Euonymus peach leaves by agarose gel electrophoresis.

[0033] (2) Screening of primers

[0034] OPA-16 was selected as a primer, and the nucleotide sequence of OPA-16 was AGCCAGCGAA (SEQ ID NO. 1) to amplify the genomic DNA of Euonymus peach leaf.

[0035] (3) Optimization of RAPD reaction system

[0036] The RAPD reaction system was determined to be 20 μL. In order to determine the optimal conditions for the RAPD reaction of Euonymus peach l...

Embodiment 2

[0060] Application example of RAPD reaction system of Euonymus peach leaf

[0061] Based on the RAPD optimal reaction system of Euonymus peach leaf described in Example 1, using OPA-16 as a primer, the nucleotide sequence of OPA-16 is AGCCAGCGAA (SEQ ID NO.1), according to Example 1 The specific steps of PCR amplification and detection of amplification products were used to amplify the genomic DNA of 10 young leaves of Euonymus peach leaves.

[0062] Depend on Figure 7 It can be seen that the 10 young leaves of E. peach leaves were effectively amplified, and the amplified bands were clear and complete, that is, the established RAPD reaction system of E. peach leaves was stable and suitable for the research of E. peach leaves.

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Abstract

The invention provides a RAPD reaction system of Euonymus peach leaf, belonging to the technical field of biological molecular marker. MgCl 2 2.50~2.70mmol / L, dNTP 0.10~0.15mmol / L, Taq DNA polymerase 1.75~2.50U, the balance is ddH 2 O. The band amplified by the RAPD reaction system of Euonymus peach leaf established by the invention is clear and complete, and has high stability, which makes up the blank of domestic research on the genetic diversity of Euonymus peach leaf.

Description

technical field [0001] The invention relates to the technical field of biological molecular markers, in particular to a RAPD reaction system and a kit of Euonymus peach leaf. Background technique [0002] Euonymus bungeanus Maxim., also known as Silk Cottonwood, Mingkai Yehe and Baidu, is a small deciduous tree of the genus Euonymus L. in the family Celastraceae. Value, is an excellent landscaping tree species. In addition, the wood of the peach leaf euonymus is a high-quality material for making utensils and dense carvings. The bark contains hard rubber, and the oil content of the seeds is as high as 40%. The leaves can replace tea, and flowers, fruits and roots can be used as medicine. Gan Ping can cure impetuousness, boredom, insomnia, pulmonary carbuncle, swelling, fracture and other symptoms. Therefore, Euonymus peach leaf can also be used as a high-quality economic tree species. [0003] Random Amplified Polymorphic DNA (RAPD) is a molecular marker technology based o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2535/139C12Q2531/113
Inventor 赵成日李玉洁曹后男刘继生金美玉霍嘉鋆崔慕雪崔凯歌张雪
Owner YANBIAN UNIV
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