Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of photoactive artificial cell and application of photoactive artificial cell in synchronous green synthesis of pentamethylene diamine and formic acid

A technology of artificial cells and construction methods, applied in the field of artificial cells, can solve problems such as reduced atom economy, carbon loss, and high environmental pressure

Pending Publication Date: 2021-09-10
NANJING UNIV OF TECH
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CO is present in the process 2 release, leading to carbon loss and reduced atom economy; second, the solution is alkaline after decarboxylation, and a large amount of exogenous acid needs to be added to maintain the neutral environment of the biocatalytic reaction. high pressure

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of photoactive artificial cell and application of photoactive artificial cell in synchronous green synthesis of pentamethylene diamine and formic acid
  • Construction method of photoactive artificial cell and application of photoactive artificial cell in synchronous green synthesis of pentamethylene diamine and formic acid
  • Construction method of photoactive artificial cell and application of photoactive artificial cell in synchronous green synthesis of pentamethylene diamine and formic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Production of pentamethylenediamine and formic acid by novel photoactive artificial cells

[0028] 1.1 Construction of Nanomaterial A

[0029] The synthesis system is 10ml, and 10mg of lysine decarboxylase (obtained from the BL21 (DE3) / pCDF-duet-CadA strain expressing E. coli-derived L-lysine decarboxylase, has been published in patent CN201810195975.2 ) and carbonic anhydrase (purchased from Shanghai Yuanye Biotechnology Co., Ltd., item number: s10157-50mg) were dissolved in deionized water, stirred at 300 rpm for 1-2 min to make the reaction solution evenly mixed, and 40 mM cobalt ions were added to increase the stirring speed Stir at 500 rpm for 10 min, and add H with a final concentration of 5 mM. 2 O 2 , stir for 30 min, centrifuge, take part of the supernatant to measure the protein concentration, wash the precipitate with deionized water 2-3 times, and use a vacuum freeze dryer to dry to obtain nanomaterial A.

[0030] Construction of Nanomaterial B ...

Embodiment 2

[0040] Example 2 Analysis method for comparison

[0041] The consumption of L-lysine was detected by SBA-40E dual-channel biosensor, and the concentration of 1,5-pentanediamine was determined by Agilent 1290 liquid chromatography system and Agilent TC-C18 column (4.6×250mm). Column temperature 40±1℃, flow rate 1.0mL·min -1 ; The injection volume is 10μl; the excitation wavelength of the fluorescence detector is 350nm, and the emission wavelength is 520nm. UV detector wavelength 250nm. Formic acid concentration was measured using an Agilent 1260 liquid chromatography system and an Aminex HPX-87H column (300 × 7.88 mm). Column temperature 65±1℃, flow rate 0.6mL·min -1 ; Injection volume 10μl; UV detector wavelength 210nm. Calcium carbonate is generated by adding calcium chloride solution to the reaction solution, and the quality of calcium carbonate is weighed to detect CO. 2 conversion rate. The calculation formula is as follows:

[0042]

Embodiment 3

[0044] The concentration of L-lysine hydrochloride was adjusted from 50g / l to 75-225g / l, and the rest were the same as 1.6 in Example 1. The production of pentamethylenediamine and formic acid was detected by liquid chromatography, such as figure 2 shown.

[0045] from figure 2 It can be seen that when the concentration of L-lysine hydrochloride is 50g / l, the yields of pentamethylenediamine and formic acid are the highest, which are 95% and 81.5%, respectively.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a construction method of a photoactive artificial cell and application of the photoactive artificial cell in synchronous green synthesis of pentamethylene diamine and formic acid. According to the method, based on the angle of artificial cells, the artificial cells are constructed, so that different catalytic reactions are carried out in the same nano space, rapid total capture of CO2 released by biological decarboxylation is promoted, and the yield of formic acid is increased; and secondly, acid-base-free biological decarboxylation and formic acid synchronous green synthesis are achieved through the artificial cells, the yield of formic acid is increased, and meanwhile reducing power is provided for the artificial cells through solar energy. The method is mild in reaction condition, high in selectivity and green and friendly in process, the reducing power is provided by utilizing infinite renewable solar energy, the production cost is reduced, and carbon loss in the biological decarboxylation process and inhibition of rapid alkalization of a microenvironment on formic acid synthesis are avoided on the premise of not influencing biological decarboxylation.

Description

technical field [0001] The invention relates to the field of artificial cells, in particular to a method for constructing a photoactive artificial cell and its application in synchronous green synthesis of pentamethylenediamine and formic acid. Background technique [0002] The technical route of biological decarboxylation has obvious advantages. Compared with chemical decarboxylation, the reaction conditions are milder, the selectivity is higher, and the process is also green and friendly. Most of them, such as pentamethylene diamine, butanediamine and ethanolamine, are amino acids and their derivatives. Obtained by biological decarboxylation. CO is present in the process 2 release, resulting in carbon loss and reduced atom economy. Second, the solution is alkaline after decarboxylation, and a large amount of exogenous acid needs to be added to maintain a neutral environment for biocatalytic reaction. high pressure. [0003] Lysine decarboxylase (CadA) can directly conve...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N11/089C12N11/04C12N11/02C12P13/00C12P7/40
CPCC12N11/089C12N11/04C12N11/02C12N15/52C12N9/88C12N9/0008C12P13/001C12P7/40C12Y401/01018C12Y402/01001C12Y102/01002Y02P20/133Y02P20/59
Inventor 李辉曾金磊陈可泉李春秋陆秋豪陈旭杨继宇
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com