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Method for detecting potassium isotope in plant

A potassium isotope and detection method technology, applied in the field of detection, can solve the problem that the source cannot be completely determined

Pending Publication Date: 2021-09-07
CHINA UNIV OF GEOSCIENCES (BEIJING) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the migration and transformation process of K in plant growth and development, as well as the source of K are not completely determined

Method used

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  • Method for detecting potassium isotope in plant
  • Method for detecting potassium isotope in plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A method for detecting potassium isotopes in plants, comprising the following steps:

[0056] S1: Collect a spinach sample and weigh 25 mg;

[0057]The plant sample was placed in a digestion tank to digest the plant sample with nitric acid, the digestion temperature was 180° C., and the digestion time was 48 hours.

[0058] The substance obtained after digestion was evaporated to dryness, then dissolved with hydrochloric acid, evaporated to dryness again and then redissolved, the operation was repeated twice, and finally 5 ml of sample solution was obtained.

[0059] S2: The potassium isotope in the sample solution is separated by chromatographic column purification using a cation exchange resin to obtain a potassium isotope solution;

[0060] Before purifying the chromatographic column, clean the column. Specifically, rinse the resin with 20 mL of hydrochloric acid with a concentration of 6M ol / l, and then rinse the resin with 10 mL of ultrapure water to ensure that t...

Embodiment 2

[0074] A method for detecting potassium isotopes in shrub leaves, comprising the following steps:

[0075] S1: Collect shrub leaf samples and weigh 25 mg;

[0076] The plant sample was placed in a digestion tank to digest the plant sample with nitric acid, the digestion temperature was 200° C., and the digestion time was 36 hours.

[0077] The substance obtained after digestion was evaporated to dryness, then dissolved with hydrochloric acid, evaporated to dryness again and then redissolved, the operation was repeated 3 times, and finally a 5ml sample solution was obtained.

[0078] S2: The potassium isotope in the sample solution is separated by chromatographic column purification using a cation exchange resin to obtain a potassium isotope solution;

[0079] Before purifying the chromatographic column, clean the column. Specifically, rinse the resin with 20 mL of hydrochloric acid with a concentration of 6M ol / l, and then rinse the resin with 10 mL of ultrapure water to ensu...

Embodiment 3

[0093] A method for detecting potassium isotopes in laver, comprising the following steps:

[0094] S1: Collect a laver sample and weigh 30 mg;

[0095] The plant sample was placed in a digestion tank to digest the plant sample with nitric acid, the digestion temperature was 190° C., and the digestion time was 40 hours.

[0096] The substance obtained after digestion was evaporated to dryness, then dissolved with hydrochloric acid, evaporated to dryness again and then redissolved, the operation was repeated 3 times, and finally 4 ml of sample solution was obtained.

[0097] S2: The potassium isotope in the sample solution is separated by chromatographic column purification using a cation exchange resin to obtain a potassium isotope solution;

[0098] Before purifying the chromatographic column, clean the column. Specifically, rinse the resin with 20 mL of hydrochloric acid with a concentration of 6M ol / l, and then rinse the resin with 10 mL of ultrapure water to ensure that t...

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Abstract

The invention relates to a method for detecting potassium isotope in a plant, which comprises the following steps: S1, digesting a plant sample, and removing impurities to obtain a sample solution; S2, separating the potassium isotope in the sample solution through chromatographic column purification to obtain a potassium isotope solution; S3, evaporating the potassium isotope solution to dryness to obtain a potassium isotope solid sample, and redissolving the potassium isotope solid sample by adopting a nitric acid solution to obtain a potassium isotope to-be-detected solution; and S4, carrying out potassium isotope composition and content detection on the potassium isotope to-be-detected solution. According to the method, the potassium isotope in the plant is detected, the detection data can be applied to tracing the migration and transformation of the potassium in the plant, the migration and transformation conditions of the potassium in the plant growth and development process are traced, and the identification index system of the potassium in the plant is perfected.

Description

technical field [0001] The invention relates to the detection field, in particular to a method for detecting potassium isotopes in plants. Background technique [0002] Potassium (K) is an important pro-life element. Living organisms are generally rich in potassium, especially in plant cells, where potassium is the metal element with the highest content. K can promote photosynthesis, facilitate protein synthesis, accelerate the flow of assimilates to storage organs, enhance stress resistance and disease resistance, and increase production. K is more than other cations in plants, and can pass through biomembranes and participate in metabolic reactions, especially in energy conversion (ATP synthesis) and energy transfer. However, the migration and transformation process of K in plant growth and development, as well as the source of K are not completely determined. Therefore, it is necessary to develop a new identification index system to trace the migration and transformati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/12
CPCG01N30/02G01N30/06G01N30/12G01N2030/065
Inventor 韩贵琳张倩
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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