In-vitro dissolution method of cross-linked sodium hyaluronate gel

A cross-linked hyaluronic acid, in vitro dissolution technology, applied in separation methods, chemical instruments and methods, filtration loops, etc., can solve the problems of uneven supernatant liquid, unable to continue enzymatic hydrolysis, etc., and achieve the effect of uniform liquid

Active Publication Date: 2021-09-03
浙江美华鼎昌医药科技有限公司
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  • Application Information

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Problems solved by technology

The existing in vitro dissolution method of cross-linked sodium hyaluronate gel can filter out large particles of cross-linked sodium hyaluronate gel by filtering with a 0.45 μm microporous membrane, but this method takes the supernatant after centrifugation Liquid, it is easy to get the gel with large particles in the lower layer, making the supernatant uneven, resulting in the inability to continue enzymatic hydrolysis

Method used

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  • In-vitro dissolution method of cross-linked sodium hyaluronate gel
  • In-vitro dissolution method of cross-linked sodium hyaluronate gel
  • In-vitro dissolution method of cross-linked sodium hyaluronate gel

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Embodiment

[0020] Embodiment: a kind of in vitro dissolution method of cross-linked sodium hyaluronate gel, such as Figure 1-Figure 3 As shown, the in vitro dissolution method comprises the following steps:

[0021] Step A, prepare cross-linked sodium hyaluronate gel, Amicon Ultra-15 tube, constant temperature shaker and centrifuge;

[0022] Step B. Weigh 5g of cross-linked sodium hyaluronate gel, open the lid of the Amicon Ultra-15 tube, and put 5g of cross-linked sodium hyaluronate gel into the inner filter tube of the Amicon Ultra-15 tube. Put the resonator in the layer filter tube, the position of the resonator is the bottom of the inner layer filter tube of the Amicon Ultra-15 tube, tighten the cover and put it into the constant temperature oscillator, set the temperature to 36.0°C, and the speed to 150r / min, Stop oscillation after 24h;

[0023] Step C. After step B, take the Amicon Ultra-15 tube out of the temperature shaker, and then put it into 37°C constant temperature water;...

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Abstract

The invention discloses an in-vitro dissolution method of cross-linked sodium hyaluronate gel, and belongs to the technical field of in-vitro dissolution of cross-linked sodium hyaluronate gel. The preparation method comprises the following steps: putting cross-linked sodium hyaluronate gel into an AmicronUltra-15 tube; and enabling the AmicronUltra-15 tube to sequentially pass through a constant-temperature oscillator and a centrifugal machine, so as to realize in-vitro dissolution of the cross-linked sodium hyaluronate gel, thereby obtaining supernate. According to the technical scheme, the effect that liquid on the lower layer of the AmicronUltra-15 tube is uniform after centrifugation is achieved.

Description

technical field [0001] The invention belongs to the technical field of in vitro dissolution of cross-linked sodium hyaluronate gel, in particular to a method for in vitro dissolution of cross-linked sodium hyaluronate gel. Background technique [0002] Cross-linked sodium hyaluronate is a polysaccharide biomaterial with good biocompatibility, and has a wide range of applications in the fields of medical cosmetology and biomedical technology. [0003] The existing cross-linked sodium hyaluronate gel can only be degraded by hyaluronidase in the body, and the degraded cross-linked sodium hyaluronate slowly breaks the chain from the initial network structure and completely degrades the cross-linked sodium hyaluronate The gel time is longer. The existing in vitro dissolution method of cross-linked sodium hyaluronate gel can filter out large particles of cross-linked sodium hyaluronate gel by filtering with a 0.45 μm microporous membrane, but this method takes the supernatant aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08B01D36/00
CPCC08B37/0072C08B37/0003B01D36/003
Inventor 齐传露戴春燕杜静孙晨露蔡佳诚王传跃
Owner 浙江美华鼎昌医药科技有限公司
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