Method for adventitious bud induction and efficient plant regeneration of cassia mimosoides seedlings

A high-efficiency regeneration and ironclad grass technology, applied in the field of plant tissue culture, can solve the problems of lack of research on ironclad grass tissue culture, and achieve the effect of reducing the cost of cultivation and shortening the time

Active Publication Date: 2021-08-31
JIAYING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, research at home and abroad is mainly focused on the chemical components and pharmacology of A. chinensis, and the research on the tissue culture of A. chinensis is relatively lacking.

Method used

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  • Method for adventitious bud induction and efficient plant regeneration of cassia mimosoides seedlings
  • Method for adventitious bud induction and efficient plant regeneration of cassia mimosoides seedlings
  • Method for adventitious bud induction and efficient plant regeneration of cassia mimosoides seedlings

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Experimental program
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Embodiment 1

[0037] 1 Experimental materials

[0038] 1.1 Experimental raw materials

[0039] The Yinna Mountain in Meizhou, Guangdong Province was selected as the sampling point to collect the pods of A. chinensis, and the collected seeds with full particles and good quality were placed in sealed bags and stored at 4°C.

[0040] 1.2 Culture conditions

[0041] Add 8g / L agar and 30g / L sucrose to the 1 / 2MS basic medium, adjust the pH value to 5.80-6.00 with sodium hydroxide solution, sterilize at 121°C and 101MPa for 20min, pour the plate into a tissue culture bottle, and wait for the medium Use after cooling and solidifying. The condition of the constant temperature incubator is 12 hours of light and 12 hours of darkness, the light intensity is 2000-2500 lx, the relative humidity is 80%, and the temperature is 22°C.

[0042] 1.3 Experimental instruments and experimental reagents

[0043] The experimental instruments and experimental reagents used are shown in Table 1 and Table 2.

[0...

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Abstract

The invention discloses a method for adventitious bud induction and efficient plant regeneration of cassia mimosoides seedling. The method comprises the steps that the adventitious buds are directly induced by taking aseptic seedlings of cassia mimosoides as explants, the adventitious buds start to be differentiated in about two weeks, and a plurality of differentiated adventitious buds can be obtained on one plant within a short time; and when the adventitious buds of the cassia mimosoides grow to 2 cm or above, the adventitious buds are transferred to a rooting culture medium for rooting, and regenerated plants are formed. Compared with the traditional method for inducing calluses through tissue wounds such as stem segments, leaves and root segments of seedlings, the time for obtaining regenerated plants is greatly shortened, and the cultivation cost of the cassia mimosoides is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for inducing adventitious buds of hecklebone seedlings and efficient regeneration of plants. Background technique [0002] Cassia mimosoides Linn. (Cassia mimosoides Linn.) is a plant of the genus Cassia in the family Fabaceae, also known as lentil, and its scientific name is Cassia mimosoides. Annual or perennial subshrubs. Distributed in Guangdong, Anhui, Guangxi, Sichuan, Taiwan and other places. Many plants of the genus Cassia have been used as medicinal materials in the folk. The plants of this genus have various pharmacological activities and are used to treat various diseases, such as diarrhea. Tiejiacao was first recorded in the Ming Dynasty's "Rescue Famine Materia Medica". It has the same origin as medicine and food. The whole herb can be used medicinally. Its young stems and leaves can be made into tea to cure dysentery. Indication...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 杨亚利翟明张鲁斌张声源何佩婷戴芊芊林展雯
Owner JIAYING UNIV
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