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Test paper strip for rapidly distinguishing recent and long-term HIV infection states through serum HIV-1 antibody detection and preparation method thereof

A test strip and fast technology, applied in the biological field, can solve the problems of being unable to distinguish newly infected people from long-term infected people, and achieve the effects of shortened detection time, simple result interpretation, and simple operation

Pending Publication Date: 2021-08-27
SOUTHERN MEDICAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods mainly judge whether they are infected by detecting the presence or absence of HIV antibodies in the blood of suspicious persons, and cannot distinguish newly-infected persons from long-term infected persons.

Method used

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  • Test paper strip for rapidly distinguishing recent and long-term HIV infection states through serum HIV-1 antibody detection and preparation method thereof
  • Test paper strip for rapidly distinguishing recent and long-term HIV infection states through serum HIV-1 antibody detection and preparation method thereof
  • Test paper strip for rapidly distinguishing recent and long-term HIV infection states through serum HIV-1 antibody detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]A test strip for quickly distinguishing HIV recent and long-term infection status through serum HIV-1 antibody detection, such as figure 1 As shown, it includes a bottom plate 8 , a sample pad 1 arranged on the bottom plate 8 , a nitrocellulose membrane 3 and an absorbent paper 4 . The two ends of the nitrocellulose membrane 3 are overlapped with the sample pad 1 and the absorbent paper 4 respectively, and the end of the sample pad 1 close to the nitrocellulose membrane 3 is sprayed with a colloidal gold-labeled mouse anti-human IgG antibody to form a gold label layer 2 .

[0044] The nitrocellulose membrane 3 is provided with a second detection line 6 coated with one HIV recombinant antigen alone, a first detection line 5 coated with GP41 recombinant antigen BE23 and GP41 recombinant antigen BE33 mixedly, and a quality control of goat anti-mouse IgG antibody Line 7.

[0045] The test strip for quickly distinguishing HIV recent and long-term infection states, the coatin...

Embodiment 2

[0065] A test strip for quickly distinguishing HIV short-term and long-term infection status through serum HIV-1 antibody detection, the structure is the same as that of Example 1, and the preparation process of the test strip of this embodiment is as follows:

[0066] 1) Prepare the sample pad, sample pad 1 was purchased from Yingke Xinchuang (Xiamen) Technology Co., Ltd.;

[0067] 2) Combine mouse anti-human IgG with colloidal gold particles to form colloidal gold-labeled mouse anti-human IgG antibody, evenly spread on the gold label pad, 42ml / sheet, freeze-dry at 45°C, and set aside;

[0068] 3) Prepare the second detection line, the first detection line and the quality control line, and then put the whole at 25°C and humidity 10 ～ Under the condition of 30%, dry or dry for 18-22h;

[0069] The specific process of preparing the second detection line is: the HIV-1 recombinant antigen is diluted to a working concentration of 2.5-3.5mg / ml with coating buffer, and the second d...

Embodiment 3

[0083] The test results of Example 2 prove that the coated GP41 mixed protein will have a weak reaction with HIV recently infected serum antibodies, resulting in weak color development. This example provides different test strips.

[0084] 1) In this test example, the first detection line GP41 fusion protein was coated with BE23 and BE33 mixture. Among them, BE230.3mg / ml and BE330.45mg / ml are mixed according to the ratio of 1:1, 1:2, 1:3, 3:1, 2:1.

[0085] 2) The method of spraying gold particles and assembling test strips in this test example is the same as that of test example 1.

[0086] 3) The preparation method of the test strip of this test example is the same as that of Test Example 1.

[0087] 4) The sample loading method of this test example is the same as that of test example 1.

[0088] 5) The test result judgment method of this test example is the same as that of test example 1.

[0089] 6) We use the test results of the test kit for distinguishing short-term a...

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Abstract

The invention discloses a test paper strip for rapidly distinguishing recent and long-term HIV infection states through serum HIV-1 antibody detection and a preparation method thereof. The test paper strip comprises a bottom plate, a sample pad arranged on the bottom plate, a nitrocellulose membrane and absorbent paper, wherein the two ends of the nitrocellulose membrane are in lap joint with the sample pad and the absorbent paper respectively, the end, close to the nitrocellulose membrane, of the sample pad is sprayed with a colloidal gold labeled mouse anti-human IgG antibody to form a gold labeled layer, a first detection line, a second detection line and a quality control line are arranged on the nitrocellulose membrane, the first detection line is coated with a GP41 recombinant antigen BE23 and a GP41 recombinant antigen BE33 in a mixed manner, the second detection line is coated with an HIV recombinant antigen independently, and the quality control line is a goat anti-mouse IgG antibody. The test paper strip can be used for judging the state of HIV-1 recent infection or long-term infection, can greatly reduce the probability of misjudgment or missed judgment of single GP41 recombinant antigen coating, and is convenient to use and simple in judgment.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically, the invention relates to a test strip for quickly distinguishing HIV short-term and long-term infection status through serum HIV-1 antibody detection and a preparation method thereof. Background technique [0002] Human immunodeficiency virus (Human Immunodeficiency Virus, HIV) is a virus that infects the cells of the human immune system. It destroys the immune cells of the human body and causes the immune system to lose its resistance, resulting in various diseases. For a long time, monitoring methods such as AIDS / AIDS virus (HIV) case reports, sentinel surveillance, and behavioral surveillance have been used to detect HIV / AIDS epidemics, grasp HIV / AIDS epidemic trends, changes in high-risk behaviors, and evaluate intervention measures. The effect provides a lot of evidence. However, it is difficult to determine whether HIV-infected persons are newly infected or long-term inf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/58
CPCG01N33/56988G01N33/558G01N33/54346G01N33/587G01N2469/20G01N2333/162
Inventor 唐时幸万政伟王海鹰赵建辉王克寅孟婷婷刘兴旺
Owner SOUTHERN MEDICAL UNIVERSITY
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