Universal rpa nucleic acid isothermal amplification primers, kits and applications for bivalve mollusk-infected herpes virus oshv-1
A herpes virus, bivalve mollusk technology, applied in DNA/RNA fragments, microorganisms, recombinant DNA technology, etc., can solve the problem of inability to achieve rapid on-site detection, and achieve good specificity test results, high sensitivity, and good stability. sexual effect
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[0037] Example 1: Establishment of the test strip RPA detection method for rapid on-site detection of OsHV-1
[0038] 1. Design of RPA upstream and downstream primers and probe primers
[0039] The genome sequences of OsHV-1 variant strains KP412538.1, GQ153938.1, NC_005881.2, MG561751.2, KY242785.1, KY271630.1 and MF509813.1 were downloaded from NCBI's GenBank and compared and analyzed to determine the differences For the conserved region among OsHV-1 mutant strains, a set of primers was designed for the zero mutation conserved region, in order to detect OsHV-1 of different mutant strains. The present invention designed and synthesized six sets of primers respectively, as shown in Table 1 below.
[0040] Table 1
[0041]
[0042] 2. DNA extraction from clinical samples
[0043]All clinical OsHV-1 positive samples used in the present invention are collected and preserved by our laboratory. The TIANamp Marine Animals DNA Kit from TIANGEN was used to extract the total DNA...
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