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Application of menadione in preparation of medicine for treating esophageal squamous cell carcinoma and/or inhibiting proliferation and metastasis of esophageal squamous cell carcinoma

A technology of esophageal scale and menadione, applied in the field of biomedicine, can solve problems such as large side effects, and achieve the effects of clear side effects, inhibition of proliferation and transfer levels

Inactive Publication Date: 2021-08-17
SHANTOU UNIV MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemoradiotherapy is an important treatment for esophageal squamous cell carcinoma, but its 5-year survival rate is only about 13%, and the side effects are relatively large

Method used

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  • Application of menadione in preparation of medicine for treating esophageal squamous cell carcinoma and/or inhibiting proliferation and metastasis of esophageal squamous cell carcinoma
  • Application of menadione in preparation of medicine for treating esophageal squamous cell carcinoma and/or inhibiting proliferation and metastasis of esophageal squamous cell carcinoma
  • Application of menadione in preparation of medicine for treating esophageal squamous cell carcinoma and/or inhibiting proliferation and metastasis of esophageal squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Cell viability test (MTS test)

[0028] To measure the sensitivity of different cell lines (KYSE30, KYSE150, KYSE450 from the Japanese Collection of Research Bioresources Cell Bank; TE1, TE3 and TE5 from the Japanese CellResource Center for Biomedical Research.) to the drug menadione, the cells were digested And cultured in RPMI Medium 1640 medium containing 10% serum (purchasing manufacturer: Yingwei Jieji (Shanghai) Trading Co., Ltd., article number: 11875119, brand: GIBCO) to form a single cell suspension, count the cells, and inoculate at 96 Well plate, 10000 cells per well. The media volume per well is 100 μl. The cells were cultured for 12 hours to adhere to the wall, and then replaced with RPMI Medium 1640 medium containing 0 μM, 5 μM, 10 μM, 20 μM, 30 μM, 40 μM and 60 μM menadione respectively (purchasing manufacturer: Yingwei Jieji (Shanghai) Trading Co., Ltd. , Item No.: 11875119, Brand: GIBCO). After continuous culture for 24 hours, 20 μl of MTS (product n...

Embodiment 2

[0033] colony formation assay

[0034]In a 12-well plate, seed 500-1,000 KYSE30 or KYSE150 cells per well. After the cells adhered to the wall, add menadione at a concentration of 15 μM to RPMI Medium 1640 cell culture medium (purchased from Yingwei Jieji (Shanghai) Trading Co., Ltd., article number: 11875119, brand: GIBCO) and incubate for 12 hours . The RPMI Medium 1640 cell culture solution containing the drug was replaced with RPMI Medium 1640 medium and the cells were continued to be cultured for another 2 weeks. After washing with pre-cooled PBS at 4°C, fix with 4°C pre-cooled methanol and glacial acetic acid at a ratio of 3:1 for 20 minutes, and stain with amethyst for 15 minutes. sigma, product number: D4540-1L, brand: sigma) is the negative control, and the specific steps refer to "Riboflavin Depletion Promotes Tumorigenesis in HEK293T and NIH3T3 Cells by Sustaining Cell Proliferation and Regulating Cell Cycle-Related Gene Transcription" (Long, L., et al. J Nutr, 2...

Embodiment 3

[0041] Wound Healing Experiment

[0042] KYSE30 or KYSE150 cells (purchased from the Japanese Collection of Research Bioresources Cell Bank) were inoculated into 6-well plates and cultured in serum-free RPMI Medium 1640 medium for 12 hours. Draw a straight line with a sterile pipette tip. The cells were washed with PBS, and then cultured in RPMI Medium 1640 medium with 2% fetal bovine serum. Use the RPMI Medium 1640 medium containing 0 μM and 15 μM menadione drug to add the drug-containing RPMI Medium 1640 medium to the cell dish of culturing KYSE30 or KYSE150 cells for 0 hours and 24 hours, and then use a microscope to examine the designated area. For microscopic observation, dimethyl sulfoxide was used as a negative control. For specific steps, refer to "RAC1 inhibition reverses cisplatin resistance in esophageal squamous cell carcinoma and induces downregulation of glycolytic enzymes" (Zeng, R.J., etal. Mol Oncol, 2019.13(9):p.2010 -2030.). Using ImageJ software (Nationa...

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Abstract

The invention relates to the field of biological medicine, in particular to application of menadione in preparation of medicine for treating esophageal squamous cell carcinoma and inhibiting metastasis of esophageal squamous cell carcinoma. The invention provides application of menadione in preparation of a medicine for treating esophageal squamous cell carcinoma. According to the invention, the side effect of menadione is clearer than that of chemotherapeutic drugs, the safety of the drug is proved, and the medicine can be used for treating esophageal squamous cell carcinoma; besides, proliferation of esophageal squamous cell carcinoma cells and transplanted tumors in nude mice can be remarkably inhibited, and the metastasis level of esophageal squamous cell carcinoma cells can be inhibited.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of menadione in the preparation of drugs for treating esophageal squamous cell carcinoma and / or inhibiting its proliferation and metastasis. Background technique [0002] Esophageal squamous cell carcinoma is one of the most common malignant tumors in the world, and the prognosis of esophageal squamous cell carcinoma is extremely poor. Smoking and alcohol history, nutritional deficiencies, hot food, and intake of carcinogens are all associated with esophageal squamous cell carcinoma. Chemoradiotherapy is an important treatment for esophageal squamous cell carcinoma, but its 5-year survival rate is only about 13%, and the side effects are relatively large. It is extremely important to find a method that can effectively treat esophageal squamous cell carcinoma with few side effects. Contents of the invention [0003] In order to solve the above problems, the present i...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00A61P35/04
CPCA61K31/122A61K9/0019A61P35/00A61P35/04
Inventor 李恩民刘禄鑫谢雷许丽艳
Owner SHANTOU UNIV MEDICAL COLLEGE
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