Detection method for diagnosing Brucella abortus by using recombinant overlapping peptide

Abstract

The invention provides a detection method for diagnosing Brucella abortus by using a recombinant overlapping peptide, and relates to the technical field of veterinary drugs and detection of zoonosis. The detection method for diagnosing the Brucella abortus by using the recombinant overlapping peptide (ROP-OMP31) comprises the following steps: screening an antigen protein, namely screening the antigen protein OMP31 from Brucella abortus outer mold proteins; vaccine design, namely designing ROP-OMP31 by using a recombinant overlapping peptide technology; artificially synthesizing, namely constructing a vector, performing prokaryotic expression and performing HPLC identification; performing escherichia coli transformation and protein purification; performing activity detection on ROP-OMP31, namely feeding the mouse for 3 weeks after nasal drip, and carrying out staining to detect the activity by using acid-fast staining; performing a guinea pig skin test, establishing a guinea pig model, and performing the skin test after feeding for 45 days; and performing a bovine skin test, establishing a bovine brucellosis model, and performing out the skin test. According to the present invention, the OMP31-ROP design comprises more antigen T cell epitopes; the protein detection purity is higher, and the corresponding quality is better; and meanwhile, compared with a PCR (Polymerase Chain Reaction) technology and an Elisa, the method is easy to operate, safer and lower in cost.

Description

technical field [0001] The invention relates to the field of zoonotic veterinary medicine and detection technology, in particular to a detection method for diagnosing Brucella bovis by using recombinant overlapping peptides. Background technique [0002] Bovine brucellosis is a zoonotic infectious disease that seriously endangers the cattle industry, food safety and public health. In developed countries, bovine brucellosis is well controlled and the incidence rate is low, but it still has a certain prevalence in developing countries; the control strategy of cattle is quarantine and culling, so the detection technology has become an important factor affecting bovine undead bacillosis. important factor controlling the effect. [0003] At present, Elisa and PCR are mainly used as detection methods at home and abroad, but this method is limited by laboratory conditions, and operation in different environments has a great risk of infection. Therefore, the detection of Brucella ...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a detection method for diagnosing Brucella bovine by using recombinant overlapping peptides, which solves the problem that bovine Brucellosis is difficult to detect

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