Aflatoxin M1 detection method and application thereof

A technology of aflatoxin and detection kit, which is applied in biochemical equipment and methods, microbe determination/inspection, DNA/RNA fragments, etc. It can solve the problem of less application of detection of small molecule compounds and achieve good applicability, Good specificity and high capture rate

Active Publication Date: 2021-08-10
SUN YAT SEN UNIV
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Problems solved by technology

[0005]Dynamic light scattering is an optical technique used to measure the hydrodynamic diameter of particles. There are fewer detection applications for molecular compounds

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  • Aflatoxin M1 detection method and application thereof
  • Aflatoxin M1 detection method and application thereof
  • Aflatoxin M1 detection method and application thereof

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[0059] In order to make the object, technical solution and technical effect of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific implementations described in this specification are only for explaining the present invention, not for limiting the present invention.

[0060] The experimental materials and reagents used, unless otherwise specified, are conventional consumables and reagents that can be obtained from commercial channels.

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Abstract

The invention discloses an aflatoxin M1 detection method and application thereof. The aflatoxin M1 detection method comprises the following steps: (1) preparing a nanoparticle probe; (2) mixing a detection sample with the aptamer, and adding the t-DNA for amplification to obtain an amplification product; and (3) mixing the nanoparticle probe obtained in the step (1) with the amplification product obtained in the step (2), and detecting the particle size change condition of the nanoparticle probe before and after mixing. According to the present invention, the N-SDA and DLS detection method has characteristics of simple operation, high sensitivity, detection limit of 1.3 pmol / L, specific response to AFM1 detection, no interference from other toxins, and good applicability in dairy products, and provides a new approach for AFM1 analysis and detection.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for detecting aflatoxin M1 and an application thereof. Background technique [0002] Mycotoxins are a class of toxic secondary metabolites produced by fungi that contaminate a variety of foods and animal feed. Due to their toxic and carcinogenic properties, mycotoxins have posed a serious health threat to humans. [0003] Aflatoxin is one of the main toxins produced by Aspergillus flavus and Aspergillus parasiticus. Among the aflatoxins, aflatoxin B1 (AFB1) is the most toxic and influential. When animals ingest feed contaminated with AFB1, AFB1 will undergo hydroxylation in the animal body to metabolize aflatoxin M1 (AFM1). Therefore, AFM1 mainly exists in the in vivo environment, such as milk and blood of animals, and can exist in various dairy or blood products due to the high stability of these animal-derived materials during food processing. Although ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6844C12N15/11
CPCC12Q1/6844C12Q2525/205C12Q2563/137C12Q2537/1376C12Q2521/301C12Q2533/101C12Q2563/155
Inventor 凌连生李婷婷黄文秀
Owner SUN YAT SEN UNIV
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