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Lactarazulene as well as preparation method and application thereof

A technology of lactobulin and milk mushroom, which is applied in chemical instruments and methods, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problems of skin rash, asthma, cardiovascular damage, etc., and achieves expansion of types and applications. , the effect of excellent anti-inflammatory activity

Active Publication Date: 2021-08-03
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-steroidal anti-inflammatory drugs are mainly used to treat chronic inflammation, but they can also cause damage to the gastrointestinal tract, kidneys, liver and cardiovascular system, and may cause skin rashes and asthma

Method used

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  • Lactarazulene as well as preparation method and application thereof
  • Lactarazulene as well as preparation method and application thereof
  • Lactarazulene as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The present invention provides a preparation method of the lactazulene described in the aforementioned embodiment, comprising: extracting milk mushrooms containing the lactazulene to form the lactazulene.

[0033] Specifically, the milk mushroom containing the said milk azulene is a damaged Russulaceae milk mushroom, and the inventor guessed that the milk azulene was produced after the damage of the Russulaceae milk mushroom, in order to repair the damaged part or after damage The compound is formed in response to stress. In short, no matter what the reason is, the milk azulene can only be extracted after the Russulaceae milk mushroom is damaged or damaged. If the Russulaceae milk mushroom is not damaged, the milk azulene cannot be detected and cannot be extracted. To obtain the lactazulene. Wherein, the above-mentioned Russulaceae milk mushroom can be orange red milk mushroom or red juice milk mushroom.

[0034] Of course, it is understandable that if the milk mushro...

Embodiment 1

[0052] The embodiment of the present invention provides a preparation method of lactazulene, comprising:

[0053] (1) Grinding 1.3Kg of fresh milk mushroom fruiting bodies with a tissue crusher, and then standing at room temperature for 3 hours;

[0054] (2) further pulverize the minced milk mushroom fruiting body in the above (1), then, use acetone (0.5L) room temperature leaching 3 times, each time for 24 hours, combine the leaching solution of each acetone leaching , continue to use the mixed solution (0.5L) of methanol: chloroform of 1:1 by volume to extract the fruiting body of Lactarius rufica after the above-mentioned leaching for 2 times, each time for 24 hours, collect and combine the extracts of each extraction, Then combine the above extracts and extracts to form an extract, and then remove organic solvents such as acetone, methanol, and chloroform by rotary evaporation to obtain a crude extract, completely dissolve the crude extract with ethyl acetate, and add an e...

experiment example 1M

[0063] Experimental example 1MTS experiment

[0064] The MTS experiment was used to determine the effect of different concentrations of lactazurin on the activity of macrophage RAW264.7, and the specific experimental method was as follows:

[0065] Use trypsin to make a homogeneous suspension of macrophage RAW264.7 in the logarithmic growth phase, and adjust the concentration to 6x10 4 Cells / mL, add 100 μL of cell suspension in 96-well plate, 37°C, 5% CO 2 Cultivated in the incubator for 24 hours, the control group was added with solvent control, and the experimental group was added with the lactazulin of Example 1 with a final concentration of 25 μg / mL, 50 μg / mL and 100 μg / mL, and each group of experiments was set up for 5 repeated wells. Repeat 3 times, add 20 μL of MTS after 24 hours of incubation, add 20 μL of 10% SDS after 2 hours of dark incubation to terminate the reaction, and measure the OD value of each well on a microplate reader.

[0066] For experimental results...

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Abstract

The invention relates to the technical field of natural extracts, in particular to lactarazulene and a preparation method and application thereof. The structural formula of the lactarazulene is shown in the specification. The lactarazulene is extracted from lactarius deliciosus of russulaceae, has excellent anti-inflammatory activity and can be used for preparing anti-inflammatory drugs.

Description

technical field [0001] The present invention relates to the technical field of natural extracts, in particular to lactazulene, its preparation method and its application. Background technique [0002] Russula lactaricus is a large-scale fungus of Russulaceae and Lactariaceae, which are both edible and medicinal. It is widely distributed in Hunan, Anhui, Fujian, Sichuan, Hubei, Guangxi and other places in my country. Russula miltiorrhiza contains polysaccharides, terpenes, sterols, polyphenols and fatty acids and other bioactive components. Terpenoids are hydrocarbons and their derivatives whose molecular formula is a multiple of isoprene in nature. Terpenoids are named and classified according to the number of isoprene, such as monoterpene (10 carbons), sesquiterpene Terpenes (15 carbons), diterpenes (20 carbons), sesquiterpenes (25 carbons), triterpenes (30 carbons) and tetraterpenes (35 carbons). Common terpenoids include: monoterpene borneol and camphor in Lauraceae, se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C13/52C07C7/00C07C7/10C07C7/12A61P29/00A61K31/015
CPCC07C13/52A61P29/00Y02A50/30
Inventor 任佳丽杨亚兰钟平胜贺雅雯杨桥罗非君付湘晋
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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