Gold-labeled rapid detection card for detecting CGMMV and preparation method thereof

Abstract

The invention discloses a gold-labeled rapid detection card for detecting CGMMV and a preparation method thereof. The gold-labeled rapid detection card comprises a shell and a detection card; an observation window and a sample adding hole are formed in the shell; the detection card comprises a bottom plate, a sample pad, a marking pad and a nitrocellulose membrane, the marking pad is coated with a probe I, and the probe I is marked by colloidal gold; a detection line T and a quality control line C are arranged on the nitrocellulose membrane at intervals; the detection line T is coated with a probe II, and the probe II is marked by sulfydryl; and the quality control line C is coated with a BSA antibody. Compared with the existing PCR detection method, the gold-labeled rapid detection card for detecting the CGMMV and the preparation method thereof have the advantages that the operation is simple, special instruments and equipment are not needed, PCR amplification is not needed, and the CGMMV in a sample can be rapidly and sensitively detected on site.

Description

technical field [0001] The invention relates to a gold standard quick test card and a preparation method thereof, in particular to a gold standard quick test card for detecting CGMMV and a preparation method thereof. Background technique [0002] Cucumber green mottle mosaic virus (CGMMV) belongs to the Tobamovirus genus of the family Tymoviridae, and mainly infects Cucurbitaceae crops, causing leaf mottle, chlorosis and deformity, etc. symptom. At present, CGMMV has been found in nearly 20 countries around the world. Since the first watermelon CGMMV outbreak in Gaizhou City, Liaoning Province in my country in 2005, so far, Guangxi, Guangdong, Liaoning, Shandong, Hubei, Hunan, Yunnan, Henan , Gansu and Shanghai have reported the occurrence of cucumber green mottled mosaic disease. Since 2012, our laboratory has investigated CGMMV on cucurbit crops in Jiangsu for several consecutive years. It also shows that CGMMV has been colonized in Jiangsu, and there are more than 20 cou...

AI Technical Summary

Problems solved by technology

Since 2012, our laboratory has investigated CGMMV on cucurbit crops in Jiangsu for several consecutive years. It also shows that CGMMV has been colonized in Jiangsu, and there are more than 20 counties / cities / districts in southern, central and northern Jiangsu. distribution, indicating that CGMMV disease has been spreading in my country, causing serious damage and economic loss to the production of cucurbit crops in my country

Early biological assay methods mainly played an important role in the study of virus strains, hosts, symptoms, etc., but were gradually abandoned due to their low accuracy and stability; serological methods established Dot-ELISA based on polyclonal antibodies ACP-ELISA and TAS-ELISA methods were established based on monoclonal antibodies. This method is easy to operate and suitable for the detection of large quantities of samples, but the detection sensitivity is limited and has certain limitations.

With the rapid development of molecular biology, molecular biology detection technology has gradually matured, not only conventional RT-PCR, but also IC-RT-PCR, MNP-RT-PCR, real-time fluorescent RT-PCR, RPA, etc., these methods The detection sensitivity and specificity are high, but the detection reagents are relatively expensive, and the professional requirements for detection are high, so it is not suitable for large sample volume detection.

In addition, extended chips, fluorescence in situ hybridization and other detection technologies have higher professional requirements, and are not suitable for popularization and application.

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