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Skin aging protein marker-ANT3 protein and non-invasive extraction method thereof

A skin aging and skin technology, applied in the field of molecular biology, to achieve broad market prospects and anti-aging effects

Pending Publication Date: 2021-07-20
杨森
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no precedent for using SERPINC1 to assist in judging the degree of skin aging

Method used

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  • Skin aging protein marker-ANT3 protein and non-invasive extraction method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0083] Using a relatively close living environment and healthy people as samples, skin samples from the curved side of the forearm were collected non-invasively (using 3M adhesive skin for sampling).

[0084] The present invention is to the measuring method of different protein content in experimenter's skin sample, according to the following steps:

[0085] (1) HPLC

[0086] Obtaining samples: 3M medical adhesive tape was pasted on the curved side of the forearm to exclude photoaging factors. (3M medical adhesive tape is 2.4cm, manufacturer: 3M Health Care 3M Brookings.), after 1 minute, gently peel off the 3M adhesive tape to ensure that the subject's skin is not damaged, and obtain a tape-like skin sample;

[0087] (1) Cut the tape-like skin sample into small pieces (0.5 x 0.5 cm) with a sterile razor blade and deposit on a glass plate before transferring to a 1.5 ml centrifuge tube; (2) Add appropriate amount of lysis buffer sample without SDS , add 2mM EDTA, 1XCocktail,...

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Abstract

The invention provides a skin aging protein marker-ANT3 protein and a non-invasive extraction method thereof. According to the present invention, the SERPINC1 (ANT3 protein) content in the skin of people of various ages is detected, and the SERPINC1 expression is up-regulated along with the age growth so as to assist the judgment of the skin aging degree, such that the skin aging is intervened in advance before the appearance of the skin aging, and the aging is effectively delayed. The method is simple and easy to implement and has a wide market prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a skin aging protein marker-ANT3 protein and a non-invasive extraction method thereof. Background technique [0002] As the human body grows older, the skin appears aging. If the skin aging is severe, it will appear very old. No one wants to see themselves older than their peers. Therefore, beauty or medical beauty has attracted more and more attention. Correctly judging the degree of skin aging and judging whether the biological age of skin aging is consistent with the actual age are the prerequisites for cosmetology or medical cosmetology. Only by finding the inner root cause of accelerated skin aging caused by aging can provide direction for cosmetology or medical cosmetology. [0003] The SERPINC1 gene is located at 1q23-25.1, spans 13.4kbp, and contains 7 exons. Antithrombin III encoded by this gene belongs to the family of serine protease inhibitors. The physi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N1/28G01N1/34G01N30/02G01N30/06G01N30/72G16B20/00
CPCG01N1/28G01N1/286G01N1/34G01N30/02G01N30/06G01N30/72G01N33/6881G01N2333/47G01N2800/7042G16B20/00
Inventor 杨森张学军张博
Owner 杨森
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