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Freeze-dried powder preparation for hair regeneration and healing as well as preparation method and application thereof

A technology of freeze-dried powder and hair, applied in the field of hair repair, to improve hair quality, repair damaged cells, and promote differentiation and proliferation

Pending Publication Date: 2021-07-20
郑州市和沐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are already many toiletries on the market that claim to have the function of hair growth, these products often only have the effect of nourishing the scalp, and the effect of hair regeneration is too obvious, and it is difficult to meet people's urgent needs for hair growth

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] A method for preparing a freeze-dried powder preparation for hair regeneration, comprising:

[0024] Step 1. Take the available healthy umbilical cord, wash and peel off the Wharton's jelly, cut the Wharton's jelly into small pieces and spread it flat, add the culture medium into the culture container and place it in the incubator for cultivation. Collect after 13-15 days of cultivation P0 generation cells.

[0025] Specifically, healthy umbilical cords can only be used with the consent of the ethics committee and pregnant women. When cleaning the umbilical cord, first wash the umbilical cord once with 75% alcohol, and then rinse the blood on the umbilical cord with normal saline; Cut the umbilical cord into small pieces of 2cm, and then peel off the artery and vein of each section of the umbilical cord, and use forceps to peel off to obtain Wharton's jelly; then cut Wharton's jelly into 2mm 3 The small pieces are paved and then placed in the culture container. The cul...

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PUM

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Abstract

The invention relates to a freeze-dried powder preparation for hair regeneration and healing as well as a preparation method and application thereof. The preparation method comprises a step 1 that an available healthy umbilical cord is taken and cleaned, Wharton's jelly is stripped and cut into small pieces, the small pieces are spread in a culture container, a culture solution is added, the culture container is placed in an incubator for culture, and P0-generation cells are collected after culture is conducted for 13-15 days; a step 2 that the P0-generation cells are put into an incubator according to the density of 1.4 million cells per bottle, and subjected to subculture for 3 days; and a step 3 that culture is terminated when the the fusion degree of the cells reaches 90% during culturing the P0 generation cells, a supernate liquid is collected and continuously freeze-dried in a freeze dryer for 50-54 hours to obtain the freeze-dried powder. The stem cell supernate contains various growth factors, so that hair follicles can be induced to be converted into a growth period from a resting period, hair growth can be promoted, and the problem of alopecia can be fundamentally solved.

Description

technical field [0001] The invention relates to the technical field of hair restoration, in particular to a freeze-dried powder preparation for hair regeneration and its preparation method and application. Background technique [0002] People's work pressure increases subsequently, and alopecia has become a kind of general social problem, and the crowd of alopecia is also more and more, has brought great distress to people. [0003] Studies have shown that the growth state of hair follicles is divided into four periods: the early growth period, the growth period, the regression period and the rest period. During the anagen period, the function of the hair follicle is active, the cell division at the bottom of the hair bulb is vigorous, and the cells produced by the meridian are continuously upward. When the hair follicle is growing, the normal growth of the hair can be guaranteed. Generally speaking, about 90% of the hair follicles are in the growth period. Too many hair f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/19A61P17/14C12N5/0775
CPCA61K35/28A61K9/19A61P17/14C12N5/0665C12N2509/00C12N2509/10
Inventor 姜南龙婕
Owner 郑州市和沐生物科技有限公司
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