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Composite microbial preparation, preparation method and application

A technology of compound microorganisms and compound preparations, applied in the field of microorganisms, can solve the problems of disease prevention and growth promotion, limited growth promotion effect, environmental pollution, pesticide residues and pathogenic bacteria resistance, etc., so as to promote the healthy growth of plants and prevent and control the occurrence of anthracnose. , the effect of increasing the income of fruit farmers

Active Publication Date: 2021-07-16
金禾佳农(北京)生物技术有限公司
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Problems solved by technology

The prevention and control of citrus diseases in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and resistance of pathogenic bacteria, which are difficult to solve.
Although microbial agents for the prevention and treatment of citrus diseases have gradually emerged, their disease prevention and growth-promoting and growth-promoting effects are mostly limited. Therefore, the citrus industry is in urgent need of a microbial agent that has a good control effect on citrus diseases.

Method used

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  • Composite microbial preparation, preparation method and application
  • Composite microbial preparation, preparation method and application
  • Composite microbial preparation, preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0027] The screening and cultivation of each bacterial strain that embodiment 1 prepares microbial agent

[0028] (1) Screening of Bacillus subtilis JNKC001

[0029] Take 10 g of fresh anthracnose-infected shatangju leaves and grind them into a paste, add them into a sterilized Erlenmeyer flask filled with 90 ml of sterile water, shake fully at 180 r / min for 30 min, and take the supernatant. Mix the liquid of each strain screened in the soil with the anthracnose juice in equal volume for 15 minutes, use the anthracnose juice as a positive control, and inoculate with the local dead spot method, select the middle leaves with the same growth, and use the conventional friction inoculation method, the left is the treatment, The right side is the control, each process 5 leaves, repeat 3 times, make a mark, observe and record the disease of the leaves. The strain with the least incidence was selected as Bacillus subtilis JNKC001.

[0030] (2) Cultivation of Bacillus subtilis JNKC00...

Embodiment 2

[0050] The preparation of embodiment 2 compound microbial preparations

[0051] 1) Set the concentration to 3*10 9 cfu / ml of Bacillus subtilis (Bacillus subtilis) JNKC001 and 1.5*10 8 Trichoderma harzianum (Trichoderma harzianum) JNHC of cfu / ml was mixed according to the ratio (volume ratio) of 10:2, and compounded to obtain the stock solution of the compound preparation;

[0052] 2) adding 6-10% of humic acid, 70-80% of adsorbent, 10-15% of wetting agent, 3-5% of stabilizing agent and 1-2% of protecting agent to the prepared stock solution to obtain a compound microbial preparation.

[0053] The possible composition of the auxiliary agent in the compound preparation can also be expressed in tabular form (per 100g compound preparation), as shown in Table 1:

[0054]

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Abstract

The invention discloses a composite microbial preparation, which comprises a bacillus subtilis JNKC001 bacterial liquid, a trichoderma harzianum JNHC bacterial liquid and an auxiliary agent, and the ratio of the total bacterial count of the trichoderma harzianum to the total bacterial count of the bacillus subtilis in the prepared composite microbial preparation is (1-3):(100-300). The compound microbial preparation can effectively improve the content of chlorophyll in citrus leaves; the citrus anthracnose is effectively prevented and controlled; the content of soluble solids in the citrus is increased; the Vc content of the citrus is increased; and the composite microbial preparation has good growth promoting and yield promoting effects on the citrus.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microbial preparation, preparation method and application. Background technique [0002] Citrus is the world's largest fruit and the third largest agricultural product in the world. In recent years, the market demand for citrus in various regions has increased year by year, and the corresponding planting area has also increased year by year. However, due to the increase in planting area, citrus leaf yellowing and diseases such as anthracnose are becoming more and more prominent. As an excellent variety of citrus, Shatangju has a relatively large planting area, and the problem that restricts the development of the citrus industry is more obvious in Shatangju. The prevention and control of citrus diseases in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as env...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/38A01P3/00A01P21/00C12R1/125C12R1/885
CPCC12N1/20C12N1/14A01N63/22A01N63/38
Inventor 张丽霞
Owner 金禾佳农(北京)生物技术有限公司
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