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Preparation method of D pantoic acid lactone with high conversion rate

A technology with high conversion rate of pantotolactone, which is applied in the field of preparation of high conversion rate D pantotolactone, can solve the problems of affecting conversion rate, low separation efficiency, environmental pollution, etc., and achieve conversion efficiency and conversion degree High, simple extraction, simple process effect

Inactive Publication Date: 2021-07-13
ANHUI HUAHENG BIOTECH +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there are following defects: the one, there are many split steps, and the separation efficiency is low; the second is that a large amount of pigment and other impurities will be produced in the chemical racemization process, and the D pantolactone obtained by splitting has a darker color and poor appearance quality The third is that chemical racemization will produce a large amount of sulfate, forming solid waste and causing environmental pollution
However, glucose dehydrogenase is easy to generate by-products during the reaction, which makes the separation of D-pantolactone difficult and affects the conversion rate.

Method used

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  • Preparation method of D pantoic acid lactone with high conversion rate
  • Preparation method of D pantoic acid lactone with high conversion rate
  • Preparation method of D pantoic acid lactone with high conversion rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of D pantolactone

[0051] Weigh 1600gDL pantolactone, 400g ammonium formate, 11.5g ammonium dihydrogen phosphate, add water until the volume of the mixed solution is 10L, stir to dissolve, adjust the pH of the solution to 6.0 with 20-25% ammonia water, heat up to 30°C, add L Pantolactone dehydrogenase, ketopantolactone reductase, and formate dehydrogenase, so that the total enzyme activity in the mixed solution is 8U, and the mixed solution is placed at a constant temperature of 37°C and stirred for 20 hours to obtain a reaction solution . The reaction solution was carried out chromatographic detection, the results are shown in figure 1 . During the reaction, samples were taken every 2 hours to detect and calculate the conversion rate, the results are shown in Figure 6 . The ee value of the reaction product was 99.1%.

Embodiment 2

[0052] Example 2 Preparation of D pantolactone

[0053]Weigh 1600gDL pantolactone, 400g ammonium formate, 23g sodium dihydrogen phosphate, add water until the volume of the mixed solution is 10L, stir to dissolve, adjust the pH of the solution to 6.0 with 20-25% ammonia water, heat up to 30°C, add L pan Acetolactone dehydrogenase, ketopantolactone reductase, and formate dehydrogenase, so that the enzyme activity in the mixed solution is 8U, and the mixed solution is placed at a constant temperature of 37°C and stirred for 20 hours to obtain a reaction solution. The reaction solution was carried out chromatographic detection, the results are shown in figure 2 . During the reaction, samples were taken every 2 hours to detect and calculate the conversion rate, the results are shown in Figure 6 . The ee value of the reaction product was 99.3%.

Embodiment 3

[0054] Example 3 Preparation of D pantolactone

[0055] Weigh 1600gDL pantolactone, 400g ammonium formate, 46g ammonium dihydrogen phosphate, add water until the volume of the mixed solution is 10L, stir to dissolve, adjust the pH of the solution to 6.0 with 20-25% ammonia water, heat up to 30°C, add L pan Acetolactone dehydrogenase, ketopantolactone reductase, and formate dehydrogenase, so that the enzyme activity in the mixed solution is 8U, and the mixed solution is placed at a constant temperature of 37°C and stirred for 20 hours to obtain a reaction solution. The reaction solution was carried out chromatographic detection, the results are shown in image 3 . During the reaction, samples were taken every 2 hours to detect and calculate the conversion rate, the results are shown in Figure 6 . The ee value of the reaction product was 99.2%.

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Abstract

The invention relates to a preparation method of D pantoic acid lactone with high conversion rate, which comprises the following steps: an enzyme preparation is added into a substrate solution to prepare D pantoic acid lactone through conversion, the substrate is DL pantoic acid lactone and ammonium formate, and the enzyme preparation is a compound enzyme composed of L pantoic acid lactone dehydrogenase, ketone pantoic acid lactone reductase and formate dehydrogenase. The preparation method has the advantages of simplicity and convenience in operation, environment friendliness and the like.

Description

technical field [0001] The invention relates to the technical field of chemistry, in particular to a method for preparing D-pantolactone with high conversion rate. Background technique [0002] D-pantolactone is an important intermediate for the preparation of panto-compounds. The preparation of D-pantolactone by decomposing DL pantoolactone by microbial enzymatic method comprises the following steps: 1) The microbial enzyme selectively hydrolyzes D-pantoolactone or L-pantoate in DL-pantoolactone lactone, to obtain L pantoate lactone and D pantoate; 2) use organic reagents to extract the mixed solution, and realize the separation of L pantoate lactone and D pantoate; 3) D pantoate through lactonization After treatment, D pantolactone is obtained, and L pantolactone is converted into DL pantolactone through chemical racemization, and then split. But there are following defects: the one, there are many split steps, and the separation efficiency is low; the second is that a l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04
CPCC12P17/04C12N15/70C12N9/0006C12N9/18C12P41/001C12Y101/01168C12Y101/01169C12N2800/22C12N9/0008C12Y102/01002Y02A50/30
Inventor 刘磊刘树蓬周芳芳余军张大伟
Owner ANHUI HUAHENG BIOTECH
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