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Broadband time-resolved fluorescence immunoassay device and method

A time-resolved fluorescence and immunoassay technology, which is applied in the fields of time-resolved fluorescence immunoassay test systems and broadband time-resolved fluorescence immunoassay devices, and can solve the problems of error in detection results, short applicable wavelength range, and long sampling time.

Active Publication Date: 2021-07-06
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of this application is to provide a wide-band time-resolved fluorescent immunoassay device and detection method to solve the existing time-resolved fluorescent immunoassay devices that have short applicable wavelength ranges, long sampling times, and errors in detection results. question

Method used

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  • Broadband time-resolved fluorescence immunoassay device and method
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  • Broadband time-resolved fluorescence immunoassay device and method

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Embodiment 1

[0096] The invention provides a broadband time-resolved fluorescent immunoassay device, which can be used for broadband down-converting or up-converting steady-state conventional fluorescent immunoassay for multiple markers. Such as image 3 A detection principle diagram of a broadband time-resolved fluorescence immunoassay device is shown. The computer control terminal simultaneously controls the spectrum detector 2 and the pulse generator 1 to work, and the pulse generator 1 provides working pulse signals for the driving motor 5-1 and the light source 3 . The specific implementation steps of the broadband down-conversion or up-conversion steady-state routine fluorescence immunoassay based on a broadband time-resolved fluorescence immunoassay device are as follows:

[0097] Step 1. Determine the types of markers in the sample to be tested and their characteristic wavelengths as λ 1 ,λ 2 ,λ 3 ,...,λ n , select the light source 3 and the spectral detector 2 in the correspo...

Embodiment 2

[0107] The invention provides a wide-band time-resolved fluorescent immunoassay device, which can be used for simultaneous wide-band down-conversion and time-lapse spectral analysis of multiple markers. Such as image 3 A detection principle diagram of a broadband time-resolved fluorescence immunoassay device is shown. The computer control terminal simultaneously controls the spectral detector 2 and the pulse generator 1 to work, and the pulse generator 1 provides working pulses for the driving motor 5-1, the light source 3 and the spectral detector 2. The specific implementation steps of the wide-band down-conversion time-lapse fluorescence immunospectral analysis based on a broadband time-resolved fluorescence immunoassay device are as follows:

[0108] Step 1, according to the type of each marker in the sample to be tested and its characteristic wavelength is λ 1 ,λ 2 ,λ 3 ,...,λ n , select the light source 3 and the spectral detector 2 in the corresponding wavelength ...

Embodiment 3

[0126] The invention provides a wide-band time-resolved fluorescent immunoassay device, which can be used for simultaneous wide-band up-conversion and time-lapse spectral analysis of multiple markers. Such as image 3 A detection principle diagram of a broadband time-resolved fluorescence immunoassay device is shown. The computer control terminal simultaneously controls the spectral detector 2 and the pulse generator 1 to work, and the pulse generator 1 provides working pulses for the driving motor 5-1, the light source 3 and the spectral detector 2. The specific implementation steps of the wide-band up-conversion time-delayed fluorescent immunospectral analysis work based on a broadband time-resolved fluorescent immunoassay device are as follows:

[0127] Step 1, according to the type of each marker in the sample to be tested and its characteristic wavelength is λ 1 ,λ 2 ,λ 3 ,...,λ n , select the light source 3 and the spectral detector 2 in the corresponding wavelength...

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Abstract

The invention discloses a broadband time-resolved fluorescence immunoassay device and a broadband time-resolved fluorescence immunoassay method. The device comprises a light source for emitting exciting light; the device further comprises a light path module, a sample loading module, a spectrum detector and a pulse generator, the light path module comprises a light filtering assembly which is used for transmitting exciting light emitted by the light source to a marker and transmitting a fluorescence signal emitted after the marker is excited to the spectrum detector; the sample loading module comprises a driving motor and a POCT detection card; the POCT detection card is used for bearing one or more samples to be detected; the driving motor is used for driving the POCT detection card to move, so that the samples to be detected are positioned on a light path of the exciting light; the spectrum detector is used for receiving fluorescence signals; the pulse generator is connected with the driving motor, the light source and the spectrum detector and used for controlling the working time sequence of the driving motor, the light source and the spectrum detector. According to the invention, broadband luminescence spectrum measurement can be carried out on various markers at the same time, the test process is simplified, and the test time is shortened.

Description

technical field [0001] The invention relates to a time-resolved fluorescence immunoassay test system, in particular to a wide-band time-resolved fluorescence immunoassay device and analysis method, belonging to the technical field of fluorescence immunoassay. Background technique [0002] Fluorescence immunoassay technology combines the high sensitivity of fluorescence technology with the high specificity of immunological technology, providing an irreplaceable detection technology for immunology, clinical histochemistry and laboratory diagnosis. At present, in addition to the identification of bacteria, viruses, protozoa, worms and fungi and the diagnosis of related diseases, the fluorescence immunoassay analyzer is also widely used in the identification and positioning of serum antibodies, pathological antibodies, antibodies and complements, autoimmune disease diagnosis, Research fields such as the pathology of immune complexes. In the process of bioluminescent detection, ...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01G01N33/58
CPCG01N21/6428G01N21/01G01N33/582G01N2021/6439G01N2021/6463G01N2021/0112
Inventor 周山勇马恩黄丽冰杨鸿艺叶斯哲张云陈卓陈学元
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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