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Chinese eaglewood leaf extract and health wine

A technology of agarwood leaves and extracts, which is applied in the field of agarwood leaf extracts and health wines, and can solve the problems of unreported anti-Helicobacter pylori effects

Active Publication Date: 2021-07-06
广东省沉香科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Health-preserving wine refers to alcoholic products with certain prevention and treatment of diseases; it is one of the wines loved by the people; but the existing health-preserving wine has not been reported to have anti-helicobacter pylori effect. Helicobacter pylori health wine has important application value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 agarwood leaf extract

[0043] S01. 500 g of dried Agarwood leaves were crushed, then 4 L of ethanol (95% by volume) was used for heating and reflux extraction for 1.5 h, and the extract was concentrated and dried to obtain the Agarwood leaf ethanol extract;

[0044] S02. Separating and purifying the ethanol extract of Agarwood leaves with a silica gel column to obtain the Agarwood leaf extract;

[0045] Wherein, in the step S02, the specific method for separating and purifying with a silica gel column is:

[0046] Put the ethanol extract of Agarwood leaves on a silica gel column (the weight of silica gel is 30 times that of the ethanol extract of Agarwood leaves, and the mesh number of the silica gel is 200-300 mesh), first use 3 times the volume of the column volume with chloroform and A mixed solvent composed of methanol was used for elution, and the eluted part was discarded; then, a mixed solvent composed of chloroform and methanol ...

Embodiment 2

[0047] The preparation of embodiment 2 Agarwood leaf extract

[0048] S01. 500 g of dried Agarwood leaves were crushed, then 4 L of ethanol (95% by volume) was used for heating and reflux extraction for 1.5 h, and the extract was concentrated and dried to obtain the Agarwood leaf ethanol extract;

[0049] S02. Put the ethanol extract of Agarwood leaves on a silica gel column (the weight and dosage of silica gel is 30 times that of the ethanol extract of Agarwood leaves, and the mesh number of silica gel is 200-300 mesh), first use 3 times the column volume with a volume ratio of 88:12 A mixed solvent composed of chloroform and methanol was used for elution, and the eluted part was discarded; and then eluted with a mixed solvent composed of chloroform and methanol with a volume ratio of 4 times the column volume of 76:24, and the collection volume ratio was 76 : The elution position that the mixed solvent elution that the chloroform of 24 and methanol form comes off, concentrates...

experiment example 1

[0065] Experimental Example 1 Anti-Helicobacter Pylori Activity Test of Agarwood Leaf Extract

[0066] The anti-Helicobacter pylori activity of the extract of Agarwood leaves was tested by the Oxford cup method; the specific method is as follows:

[0067] Under sterile conditions, 0.3 mL of bacteria solution containing Helicobacter pylori ATCC700392 (concentration of 2.5×10 7 each / mL) into the culture medium, and spread evenly with an L-shaped smear stick (sterilized); then dry at room temperature for 8 minutes, and then put the sterilized Oxford cup into a petri dish with sterile tweezers; then Oxford Add 100 μL of liquid medicine with a concentration of 100mg / mL (containing the extract of Agarwood leaves to be tested) into the cup; after adding the liquid medicine, put the petri dish into the incubator for cultivation, and measure the inhibition zone after culturing at 37°C for 16 hours diameter.

[0068] Using the above method, test the Agarwood leaf extracts prepared in ...

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Abstract

The invention relates to the technical field of traditional Chinese medicines, and particularly discloses Chinese agilawood leaf extract and health wine. The Chinese agilawood leaf extract is prepared by the following steps: S01, crushing Chinese agilawood leaves, and carrying out extracting with ethanol to obtain Chinese agilawood leaf-ethanol extract; and S02, carrying out separating and purifying on the Chinese agilawood leaf-ethanol extract by using a silica gel column, thereby obtaining the Chinese agilawood leaf extract. Furthermore, the health wine is obtained by adding 0.5-10 grams of the Chinese agilawood leaf extract into every 1 litre of Chinese Baijiu. Researches show that the Chinese agilawood leaf extract has helicobacter pylori resisting activity. Therefore, active ingredients of the Chinese agilawood leaf extract can be further used for preparing drugs or health care products with an anti-helicobacter pylori effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to an extract of agarwood leaves and health-preserving wine. Background technique [0002] Helicobacter pylori disease is a spiral-shaped, slightly anaerobic bacterium; human Helicobacter pylori infection can cause gastritis, peptic ulcer, lymphoproliferative gastric lymphoma, etc. The International Agency for Research on Cancer of the World Health Organization lists Helicobacter pylori (infection) in a list of carcinogens. Therefore, it has important application value to develop a kind of Chinese medicinal material with anti-Helicobacter pylori effect from Chinese medicinal materials. [0003] Agarwood leaves are the leaves of Aquilaria sinensis (Lour.) Spreng., a tree of the genus Aquilaria in the family Daphneaceae; existing studies have shown that the extracts of agarwood leaves extracted by different methods have anti-inflammatory, analgesic, anti-tumor, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/835A61P31/04C12G3/055
CPCA61K36/835A61P31/04C12G3/055A61K2236/333A61K2236/55Y02A50/30
Inventor 张雄汉黄仲斌吴照初何峰杨懋勋黎钰浩叶伟
Owner 广东省沉香科学研究所
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