A method for extracting and determining sulfa antibiotics in solid-state environmental samples by water solvent

Abstract

The invention belongs to the field of antibiotic detection, and more specifically relates to a method for extracting and measuring sulfonamide antibiotics in solid-state environmental samples by water solvent. The assay method includes a sample extraction step, wherein the sample extraction step includes the following sub-steps: using pure water as an extractant, conducting the extraction of sulfonamide antibiotics at a temperature greater than or equal to 80°C and a pressure greater than or equal to 1 atmosphere. extract. The extraction is carried out under high temperature and high pressure, and there is no need to change the phase of the extract after the extraction, which not only saves time, but also avoids the inevitable loss of the target analyte during the phase change process and affects the measurement accuracy. The assay method proposed by the present invention is a green extraction method, does not involve any organic solvent in the extraction process, will not pollute the environment, and the overall operation time is also greatly shortened, and at least 10 sulfonamide antibiotics can be tested within 10 minutes. rapid separation detection.

Description

technical field [0001] The invention belongs to the field of antibiotic detection, and more particularly relates to a method for extracting and measuring sulfonamide antibiotics in solid environmental samples with water solvent. Background technique [0002] Antibiotics refer to chemical substances produced by biological action that have anti-pathogen or other types of secondary biological metabolites and other synthetic and semi-synthetic chemicals that can act on living cells. In 2016, my country's annual consumption reached 180,000 tons, and its role involves agriculture, animal husbandry, medical care, and aquaculture. With the abuse of antibiotics, the environmental problems are becoming more and more serious, and the harm caused by antibiotic residues in environmental media has become a hot spot in the 21st century. The establishment of the antibiotic residue detection method has important practical significance for the migration and transformation of antibiotics in e...

AI Technical Summary

Problems solved by technology

[0004] For the defect of prior art, the object of the present invention is to provide a kind of method simple and green and the assay method of sulfa antibiotics in the solid-state environmental sample that measurement result is accurate, and it utilizes the characteristic that sulfa antibiotics itself has certain polarity, adopts pure water As an extractant, it is extracted under high temperature and high pressure, aiming to solve the technical problems that the extractant contains organic solvents and other complex components in the existing sulfonamide antibiotic determination method, which leads to cumbersome separation and enrichment steps and affects the detection accuracy.

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