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Lactobacillus rhamnosus, fermentation lysate for regulating skin micro-ecology, preparation method and application of lactobacillus rhamnosus and fermentation lysate

A Lactobacillus rhamnosus, lysis technology, applied in skin care preparations, microorganism-based methods, microorganisms, etc., can solve the problems of lack of analysis of active substances in regulation, insufficient release of active substances, reduced activity, etc. Reduced risk of skin allergies, easy to magnify, effect with high content of active substances

Active Publication Date: 2021-06-29
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the course of the experiment, the live bacteria cells were used to test the efficacy of the bacteria, and the extracellular products of the bacteria were discarded. At the same time, the active substances in the cells could not be fully released without the wall-breaking process, and the activity was greatly reduced. As a result, this strain only has the activity of general Lactobacillus rhamnosus, such as the expression of antimicrobial peptide genes and protein levels, the regulation of pathogenic bacteria such as alba and other pathogenic bacteria, and the analysis and comparison of active substances are all lacking

Method used

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  • Lactobacillus rhamnosus, fermentation lysate for regulating skin micro-ecology, preparation method and application of lactobacillus rhamnosus and fermentation lysate
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  • Lactobacillus rhamnosus, fermentation lysate for regulating skin micro-ecology, preparation method and application of lactobacillus rhamnosus and fermentation lysate

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preparation example Construction

[0098] The present invention is not limited to the preparation of logarithmic strains, which can be prepared using common methods in the art, for example, inoculating the strains into MRS liquid medium, and culturing at 37°C for 15-20h to obtain the strains in logarithmic phase. period strains.

[0099] In one embodiment, the enzymes used in the enzymatic hydrolysis are lysozyme, dispase and helicase, preferably the specific enzyme activity of the lysozyme is 2000-20000IU / mg, and the specific enzyme activity of the dispase The activity is 50000-100000IU / g; preferably, the enzymolysis time is 1-2h, and the enzymolysis temperature is 35-40°C;

[0100] Preferably, for 1 g of the fermentation strain, the added lysozyme is 0.01-0.1 mg, the added helicase is 1-10 mg, and the added neutral protease is 0.01-0.1 mg.

[0101] In one embodiment, the supernatant obtained after centrifuging the first fermentation broth is filtered with a filter membrane to obtain the second fermentation b...

Embodiment 1

[0118] The acquisition of embodiment 1 bacterial strain

[0119] Collect newborn breast milk, dilute the breast milk 10 times and mix it with non-sterile purified water, take 100 μL and spread it on a solid MRS plate, incubate it in a 37°C incubator for 48 hours, and then separate and screen the grown single colony by dilution and coating on a solid MRS plate , 37 ° C incubator for 48 hours, the single colony morphology and physiological and biochemical experiments are as follows.

[0120] 1.1 Morphological observation

[0121] The bacterial species is spread on a glass slide, stained with Gram staining, and the morphology of bacterial cells is observed. The colony applicator is spread on the MRS solid plate and cultured overnight at 37°C for 24 hours, and the single colony state of the bacteria on the plate is observed.

[0122] 1.2 Physiological and biochemical experiments

[0123] After separation and purification, the strains were obtained for physiological and biochemi...

Embodiment 2-1

[0154] Pick Lactobacillus rhamnosus strains from the test tube and inoculate them in 500mL of sterile seed culture solution containing MRS, let it stand still, and cultivate it at 37°C for 18 hours, and in the logarithmic growth phase, inoculate again according to 0.5% inoculum amount to 5L fermentation In the culture medium, the fermentation medium contains 2 wt% of glucose, 1.5 wt% of peptone, 0.1 wt% of dipotassium hydrogen phosphate and 0.5 wt% of magnesium sulfate, cultivated at 37°C for 50h without residual sugar, and the fermentation is completed to obtain the first fermentation liquid, Denoted as fermentation broth 1.

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Abstract

The invention discloses lactobacillus rhamnosus, a fermentation lysate for regulating skin micro-ecology and a preparation method of the lactobacillus rhamnosus and the fermentation lysate. The lactobacillus rhamnosus 11-7 disclosed by the invention is preserved in the China Centre for Type Culture Collection, and the preservation number of the lactobacillus rhamnosus 11-7 is CCTCC NO:M2021185. The invention provides a fermentation lysate, which comprises the following components in percentage by mass: 0.2-1% of protein, 0.3-0.8% of polysaccharide and 0.2-1% of amino acid. The fermentation lysate obtained by fermenting the strain in the invention can strongly inhibit reproduction and growth of pathogenic bacteria, has a promoting effect on part of probiotics, and particularly has a strong inhibiting effect on candida albicans pathogenic form hyphae; and furthermore, the fermentation lysate can generate antibacterial peptide in the fermentation process, also up-regulates the expression quantity of skin cell antibacterial peptides in the gene level and the protein level, improves the skin immunity, and can significantly regulate the skin micro-ecological environment as a cosmetic raw material.

Description

technical field [0001] The invention relates to the technical field of cosmetic raw materials, in particular to a lactobacillus rhamnosus, a fermented lysate for regulating skin microecology, a preparation method and an application thereof. Background technique [0002] Skin microecology is to study the relationship between the structure and function of human skin microbiota and human skin itself, so as to explain the interaction between skin and microorganisms and the nature of abnormal changes in skin. The skin microecology is mainly composed of various microorganisms such as bacteria, fungi, viruses, mites, and arthropods, as well as tissues, cells, various secretions, and microenvironments on the skin surface. Skin micro-ecological balance is formed by the interaction of skin microbes, host and external environment. Human skin microecology is directly related to human health. When the human body microecology is abnormal, symptoms such as burning, tingling, itching, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/00C12P19/04C12P13/04A61K8/99A61P17/00A61P31/10A61P37/04A61Q17/00A61Q19/00C12R1/225
CPCC12P21/00C12P19/04C12P13/04A61K8/99A61Q17/005A61Q19/00A61P37/04A61P17/00A61P31/10A61K2800/85C12N1/205C12R2001/225
Inventor 陆震魏玉洁孙元军杜冉冉赵岩郭学平
Owner BLOOMAGE BIOTECHNOLOGY CORP LTD
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