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Application of AlGaAs/GaAs HEMT biosensor in detection of IL-1beta potential inhibitor

A biosensor and β-inhibitor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as easy recurrence, high lethality, and difficult to cure, and achieve high-sensitivity effects

Active Publication Date: 2021-06-22
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The survey results show that the prevalence of allergic rhinitis in my country has risen from 11.1% to 17.6% in just five years, and it has reached more than 20% in some cities; eczema is divided into acute, subacute, and chronic stages, which are difficult to cure , easy to relapse, mostly in infants and young children, seriously affecting the health of infants and young children; allergic purpura is one of the common hemorrhagic diseases in children, the age of onset is mostly 3-14 years old, it has a high mortality rate, and there are still difficulties in clinical treatment

Method used

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  • Application of AlGaAs/GaAs HEMT biosensor in detection of IL-1beta potential inhibitor
  • Application of AlGaAs/GaAs HEMT biosensor in detection of IL-1beta potential inhibitor
  • Application of AlGaAs/GaAs HEMT biosensor in detection of IL-1beta potential inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Preparation of an IL-1β-AlGaAs / GaAs HEMT biosensor

[0027] (1) Use the CHI-660E electrochemical workstation to measure the I of each channel of the GaAs / GaAs HEMT device DS -V DS Curve, judge and compare the performance of each channel.

[0028] (2) Place the clean AlGaAs / GaAs HEMT device in 3-mercaptopropionic acid (3-MPA) aqueous solution, soak it at room temperature, and generate Au-S bonds on the surface of the AlGaAs / GaAs HEMT device to form a self-assembled monolayer;

[0029] (3) Wash away the 3-MPA on the surface of the AlGaAs / GaAs HEMT device with deionized water, add 20mM carbodiimide hydrochloride (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDC) and 50mM N-hydroxy A mixed aqueous solution of succinimide (N-hydroxysuccinimide, NHS) with a volume ratio of 1:1 generates stable amine activated products for activating carboxyl groups;

[0030] (4) Wash the AlGaAs / GaAs HEMT device with PBS, add the macrophage migration inhibitor (IL-...

Embodiment 2

[0032] Example 2 Application of an IL-1β-AlGaAs / GaAs HEMT biosensor in the detection of the molecular interaction between IL-1β and amygdalin

[0033](1) Take the IL-1β-AlGaAs / GaAs HEMT prepared in Example 2 as the reaction device, configure the concentration range of the small molecule solution to be 0.1pM-1.0mM, and increase the concentrations from low to high to IL-1β-AlGaAs / GaAs / Add amygdalin standard solution on GaAs HEMT device, adopt electrochemical device to record the electric current intensity between source-drain (I DS ), I at different concentrations DS -V DS Signal such as figure 2 Shown; It can be seen from the figure that when the concentration range is 0.1pM-1.0μM, I DS The absolute value of the signal keeps decreasing; when the concentration exceeds 1.0 μM, I DS The signal changes irregularly;

[0034] (2) The logarithm (Lg [A] of amygdalin standard solution concentration g ]) is the abscissa, and the relative value of the current change (I-I 0 ) / I 0...

Embodiment 3

[0041] Example 3 Application of an IL-1β-AlGaAs / GaAs HEMT biosensor in the detection of the intermolecular interaction between IL-1β and 5-O-methylvisamigoside

[0042] (1) Take the IL-1β-AlGaAs / GaAs HEMT prepared in Example 2 as the reaction device, configure the concentration range of the small molecule solution to be 0.1pM-1.0mM, and increase the concentrations from low to high to IL-1β-AlGaAs / GaAs / Add 5-O-methyl visamidol glycoside standard solution on GaAs HEMT device, adopt electrochemical device to record the electric current intensity between source-drain (I DS ), I at different concentrations DS -V DS Signal such as figure 2 Shown; It can be seen from the figure that when the concentration range is 0.1pM-1.0μM, I DS The absolute value of the signal keeps decreasing; when the concentration exceeds 1.0 μM, I DS The signal changes irregularly;

[0043] (2) With the logarithm (Lg[A] of 5-O-methyl visamidol standard solution concentration g ]) is the abscissa, and ...

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Abstract

The invention provides an application of an AlGaAs / GaAs HEMT biosensor in detection of an IL-1beta potential inhibitor, and the application is characterized in that the IL-1beta-AlGaAs / GaAs HEMT biosensor is constructed by using an AlGaAs / GaAs HEMT device as a transducer and using interleukin 1beta protein modified on the HEMT device as an identification element through self-assembly of a single layer, the IL-1beta-AlGaAs / GaAs HEMT biosensor is used for identifying the IL-1beta potential inhibitor, and the IL-1beta-AlGaAs / GaAs HEMT biosensor is used for detecting the IL-1beta potential inhibitor. The detection concentration is as low as pM level, the detection range covers five orders of magnitude, and the method has high sensitivity and strong specificity.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to the application of an IL-1β-AlGaAs / GaAs HEMT biosensor in detecting IL-1β potential inhibitors. Background technique [0002] Anaphylaxis, also known as type I hypersensitivity, is the result of an abnormal immune response that triggers inflammation, vasodilation, nervousness, and more. According to statistics, about 300 million people in the world suffer from asthma, more than 500 million people suffer from allergic rhinitis, and about 400 million people suffer from eczema and atopic dermatitis. The survey results show that the prevalence of allergic rhinitis in my country has risen from 11.1% to 17.6% in just five years, and it has reached more than 20% in some cities; eczema is divided into acute, subacute, and chronic stages, which are difficult to cure , easy to relapse, mostly in infants, seriously affecting the health of infants; Henoch-Schonlein pur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/26
CPCG01N27/416G01N27/26
Inventor 马丽娟吴志生
Owner BEIJING UNIV OF CHINESE MEDICINE
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