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Preparation method and application of frozen gel scaffold

A technology of cryogel and sericin, which is applied in the field of medical biocomposite materials, can solve the problems of uneven distribution of antibacterial nanoparticles and misuse of cryogel antibiotics, and achieve the effect of rapid blood absorption and hemostasis, and excellent antibacterial activity

Active Publication Date: 2021-06-22
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above problems, the present invention provides a preparation method and application of a cryogel scaffold, which mainly solves the abuse of existing cryogel antibiotics, uneven distribution of antibacterial nanoparticles, and no method for preparing hemostatic and antibacterial cryogels through sericin And other issues

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  • Preparation method and application of frozen gel scaffold
  • Preparation method and application of frozen gel scaffold
  • Preparation method and application of frozen gel scaffold

Examples

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preparation example Construction

[0035] The preparation method of cryogel support, comprises the steps

[0036] Preparation of double bond modified sericin: Sericin powder is dry dissolved in phosphate buffer solution, methacrylic anhydride is added dropwise, and impurities are removed;

[0037] Preparation of sericin jelly gel: double bond modified sericin solution mixed with ammonium persulfate solution and tetramethylethylenediamine solution, and frozen;

[0038] Preparation of sericin cryogel scaffold: the sericin cryogel was reacted in silver nitrate solution, and freeze-dried.

[0039] Also includes

[0040] Dry preparation of sericin powder: silkworm cocoons placed in Na 2 CO 3 solution, stirred to obtain a gum silk solution, impurity removal, dialysis, and freeze-drying.

[0041] In the preparation of the double-bond modified sericin, dialysis is performed after the impurity removal step, and freeze-drying is performed to obtain the double-bond modified lyophilized sericin.

[0042] During the pr...

Embodiment 1

[0072] The preparation of embodiment 1 sericin / silver composite cryogel support

[0073] Step 1) extraction of sericin

[0074] 1. Weigh silkworm cocoons (Bombyx mori cocoons (Baiyu, Haoyue), A. mylitta or castor bean cocoons, etc.), cut them into pieces, and use ddH 2 O was cleaned, and the water was removed by centrifugation at 3500rpm for 5 minutes;

[0075] 2. Add 20mL of 0.02mol / L Na to each gram of cocoon 2 CO 3 The aqueous solution is placed in a constant temperature water bath and stirred at 100°C for 1 hour to dissolve the sericin and obtain a sericin solution;

[0076] 3. Transfer the obtained sericin solution into a 50mL centrifuge tube, and centrifuge at 3500rpm for 5 minutes to remove impurities in the sericin solution to obtain a clear solution;

[0077] 4. Transfer the clarified solution into a dialysis bag with a molecular cut-off of 3500 Da, 2 Dialyze in O for 72 hours, stir slowly, and change the water every 6 hours;

[0078] 5. Transfer the sericin sol...

Embodiment 2

[0093] Example 2 Characterization and detection of sericin cryogel and sericin / silver composite cryogel freeze-dried scaffold

[0094] figure 1 Among them, A, B, and C are the sericin cryogel (SMC), sericin / silver composite cryogel (SMC@Ag), sericin / silver composite cryogel (SMC@Ag) gel prepared in Example 1, respectively. The physical picture of the dry support.

[0095] figure 1 Middle D is the scanning electron micrograph of the freeze-dried scaffold of sericin cryogel (SMC) and sericin / silver composite cryogel (SMC@Ag) prepared in Example 1.

[0096] observe as figure 1 As shown in middle D, sericin jelly gel has a lot of loose large-scale pore structure, which is suitable as a material with rapid water absorption or blood absorption requirements. On the sericin / silver composite cryogel, there is a nano-scale spherical structure that the pure sericin cryogel does not have, that is, silver nanoparticles generated by reduction, which endow the cryogel with antibacterial ...

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Abstract

The invention belongs to the field of medical biological composite materials, and particularly discloses a preparation method and application of a frozen gel scaffold. The preparation method of the frozen gel scaffold comprises the following steps: preparation of double-bond modified sericin: dry-dissolving sericin powder in a phosphate buffer solution, dropwise adding methacrylic anhydride, and removing impurities; sericin frozen gel preparation: mixing the double-bond modified sericin solution with an ammonium persulfate solution and a tetramethylethylenediamine solution, and freezing; and preparation of the sericin frozen gel scaffold: putting the sericin frozen gel in a silver nitrate solution for reaction, and freeze-drying. The sericin / silver composite frozen gel scaffold is applied as a hemostatic material. The invention provides the novel preparation method of the sericin frozen gel scaffold, wherein the preparation method is simpler and more environment-friendly; the sericin is used as a raw material to prepare the frozen gel; the sericin frozen gel has relatively good rapid blood suction and hemostasis performance and relatively excellent antibacterial activity.

Description

technical field [0001] The invention belongs to the field of medical biocomposite materials, in particular to a preparation method and application of a cryogel support. Background technique [0002] Wound healing refers to the repair and regeneration of damaged tissues when the body is subjected to external forces. Mild trauma is generally limited to the skin, while severe trauma mainly includes severe defects of skeletal muscles, nerves and other organs, accompanied by massive blood loss and fractures. The number of trauma deaths worldwide is as high as millions every year. When trauma occurs, it is accompanied by traumatic bleeding, which is mainly divided into external bleeding and internal bleeding. Uncontrolled hemorrhage is one of the main reasons for the death of trauma patients. Trauma victims die days or weeks after their injuries, usually from infection, multi-organ failure, or devastating brain injury. Therefore, timely hemostasis and infection prevention after ...

Claims

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Application Information

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IPC IPC(8): A61L24/10A61L24/00A61L24/02
CPCA61L24/108A61L24/02A61L24/0031A61L24/0015A61L2300/104A61L2300/404A61L2400/04C08L89/00
Inventor 王琳王征朱媛媛乞超
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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