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Agrocybe salicacola and cultivation method thereof

A cultivation method and the technology of Agrocybe edodes, which are applied in the strains of Agrocybe splendens and its cultivation field, can solve the problems of lack of breeding of excellent strains and systematic research on cultivation techniques, and lack of large-scale commercial cultivation and production, and achieve The effect of excellent strains, stable properties, and high application value

Active Publication Date: 2021-06-15
SCI INST YUNNAN SUPPLY & SALE COOP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that the fungus has a simple cultivation method, a wide range of adaptation, fast fruiting, and has a great prospect for promotion. However, it has not been cultivated on a large scale and commercialized at present, which may be due to the lack of good strain selection and Systematic research on cultivation techniques is closely related to

Method used

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  • Agrocybe salicacola and cultivation method thereof
  • Agrocybe salicacola and cultivation method thereof
  • Agrocybe salicacola and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Isolation, Cultivation, Identification and Preservation of Agrocybe japonica JZ527 (CGMCC NO.20231)

[0082] 1. Isolation and cultivation of Agrocybe splendens strain

[0083] (1) Tissue separation of the collected wild Acarina syringae sporocarps, inoculating the inner wall tissue pieces of Amangus syringae on the slant of test tube agar medium, and culturing at 20-25°C for 7-10 days, and the isolated cultured test tubes Observe the records every day, remove the contaminated test tubes in time, select the non-polluted and good-growing strains, and obtain the isolated test tube species of Acromanus edulis. The test tube agar medium is a PDA comprehensive medium, and its formula is: 20% potato, 2.0% glucose, 0.2% peptone, KH 2 PO 4 0.1%, MgSO 4 0.05%, agar 2.0%, culture temperature 20-25°C, the percentages mentioned are mass fractions.

[0084] (2) Inoculate the mycelium of the isolated test tube species of Amanitatus willowii on the PDA comprehensive medium plate c...

Embodiment 2

[0112] Cultivation method of fruiting body of Agrocybe japonica JZ527 (CGMCC NO.20231)

[0113] 1. Production of willow agrocybe strains

[0114] According to the formula: 70% sawdust, 29% wheat, 1% gypsum, bottle with mixing materials, make the original seed bottle, sterilize and cool, cut the test tube with the overgrown willow mushroom JZ527 into small pieces, and place it in the dark at 25°C for cultivation , it can be used after being fully grown. According to the formula: 60% sawdust, 29% cottonseed hulls, 10% wheat bran, 1% gypsum, packed in polypropylene seed bags (size 17cm×35cm), about 800g per bag, sterilized and cooled to make cultivars, and When the original seed has grown well, it is inserted and cultivated in the dark at 25°C, and it can be used as a cultivated species after it is fully grown.

[0115] 2. Soil-covered cultivation of willow head mushroom

[0116] A. Overburden

[0117] Remove the bag of the fungus bag full of hyphae, and arrange the fungus tu...

Embodiment 3

[0127] Other steps are the same as in Example 2, except that the formula of the original seed medium is as follows: 65 parts of sawdust, 34 parts of wheat, and 1 part of gypsum;

[0128] The compost formula for cultivated species is: 50 parts of sawdust, 25 parts of cottonseed hulls, 24 parts of wheat bran, and 1 part of gypsum.

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Abstract

The invention relates to agrocybe salicacola and a cultivation method thereof. The agrocybe salicacola JZ527 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 24, 2020, with the preservation number of CGMCC No.20231. The cultivation method comprises the steps of cultivar preparation and soil covering cultivation. Agrocybe salicacola fruiting bodies in the Yunnan origin area are utilized to obtain the agrocybe salicacola, the fruiting time is long and the yield is stable when the agrocybe salicacola is cultivated in a greenhouse in an earthing manner, and an excellent strain is provided for artificial cultivation of the agrocybe salicacola.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Acarina japonica and a cultivation method thereof. Background technique [0002] Willow Acorn Mushroom ( Agrocybe salicacola ), also known as Liusheng Tiantou mushroom, willow mushroom, willow tree mushroom, is a unique and rare edible fungus in Yunnan. The fungus occurs from March to November every year, and is abundant in June to October, mainly on willows and poplars in temperate and subtropical regions. Yangliu Tiantou Mushroom is delicious, crisp and tender, and is very popular among local people. Liu Shaoxiong et al. analyzed the nutritional components of the fruiting body of A. willowa. A. willow. The fruiting body of A. willowa not only has high protein content, but also is very rich in crude fiber, minerals and amino acids, especially the essential amino acids valine, threonine, and lysine. The acid content is high, and it also contains the trace e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G18/00A01G18/20A01G18/70C12R1/645
CPCA01G18/00A01G18/20A01G18/70Y02W30/40
Inventor 杨珍福刘绍雄尚陆娥吴素蕊李建英罗孝坤李雪松
Owner SCI INST YUNNAN SUPPLY & SALE COOP
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