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Ferroptosis-inducing nano-composite, preparation method and application of ferroptosis-inducing nano-composite in tumor treatment

A nanocomposite, ferroptosis technology, applied in the field of medicine, can solve the problem of anti-tumor that has not yet been discovered, and achieve the effect of enhancing anti-tumor effect, less toxic and side effects, and simple ingredients

Pending Publication Date: 2021-06-11
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no research report on the preparation of ferroptosis inducers and anti-tumor research reports on nanocomposites loaded with artemisinin drugs and oral iron supplements using lactoferrin as a carrier

Method used

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  • Ferroptosis-inducing nano-composite, preparation method and application of ferroptosis-inducing nano-composite in tumor treatment
  • Ferroptosis-inducing nano-composite, preparation method and application of ferroptosis-inducing nano-composite in tumor treatment
  • Ferroptosis-inducing nano-composite, preparation method and application of ferroptosis-inducing nano-composite in tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation of lactoferrin loaded with artesunate-iron dextran nanocomposite for ferroptosis induction, the specific steps are as follows:

[0049] (1) Dissolve 2 mg of artesunate and 1.601 mg of iron dextran in 2 mL of dichloromethane to obtain solution 1;

[0050] (2) Disperse 10 mg of lactoferrin in 2 mL of PBS phosphate buffer (pH=7.4, 1× buffer solution) to obtain solution 2;

[0051] (3) Add solution 1 to solution 2 (solution volume ratio is 1:1), and sonicate for 10 minutes under an ultrasonic (power 250W) probe to obtain a crude nanocomposite;

[0052](4) Transfer the crude nanocomposite product to a centrifuge tube, centrifuge at 2000rpm for 3min, discard the supernatant; then add 2mL of PBS buffer for washing (pH=7.4, 1×), resuspend the precipitate, centrifuge at 2000rpm for 3min, Discard the supernatant; add 2 mL of PBS buffer to wash (pH=7.4, 1×), resuspend the pellet, 2000 rpm;

[0053] (5) The crude nanocomposite product after centrifugation was tran...

Embodiment 2

[0056] The preparation of lactoferrin loaded with artemether-ferrous fumarate nanocomposite to induce ferroptosis, the specific steps are as follows:

[0057] (1) Dissolve 2 mg of artemether and 2.28 mg of ferrous fumarate in 2 mL of dichloromethane to obtain solution 1;

[0058] (2) Disperse 10 mg of lactoferrin in 2 mL of PBS phosphate buffer (pH=7.4, 1X) to obtain solution 2;

[0059] (3) Add solution 1 to solution 2 (solution volume ratio is 1:1), and sonicate for 10 minutes under an ultrasonic (power 250W) probe to obtain a crude nanocomposite;

[0060] (4) Transfer the crude nanocomposite product to a centrifuge tube, centrifuge at 2000rpm for 3min, discard the supernatant; then add 2mL of PBS buffer for washing (pH=7.4, 1×), resuspend the precipitate, centrifuge at 2000rpm for 3min, Discard the supernatant; add 2 mL of PBS buffer to wash (pH=7.4, 1×), resuspend the pellet, 2000 rpm;

[0061] (5) The crude nanocomposite product after centrifugation was transferred to a...

Embodiment 3

[0064] The preparation of ferroptosis-induced ferroptosis-induced ferroptosis nanocomposite with lactoferrin carried by dihydroartemisinin, the specific steps are as follows:

[0065] (1) Dissolve 2 mg of dihydroartemisinin and 2.42 mg of ferrous succinate in 2 mL of dichloromethane to obtain solution 1;

[0066] (2) Disperse 10 mg of lactoferrin in 2 mL of PBS phosphate buffer (pH=7.4, 1X) to obtain solution 2;

[0067] (3) Add solution 1 to solution 2 (solution volume ratio is 1:1), and sonicate for 10 minutes under an ultrasonic (power 250W) probe to obtain a crude nanocomposite;

[0068] (4) Transfer the crude nanocomposite product to a centrifuge tube, centrifuge at 2000rpm for 3min, discard the supernatant; then add 2mL of PBS buffer for washing (pH=7.4, 1×), resuspend the precipitate, centrifuge at 2000rpm for 3min, Discard the supernatant; add 2 mL of PBS buffer to wash (pH=7.4, 1×), resuspend the pellet, 2000 rpm;

[0069] (5) The crude nanocomposite product after c...

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Abstract

The invention discloses a ferroptosis-inducing nano-composite, a preparation method and application of the ferroptosis-inducing nano-composite in tumor treatment. The preparation method comprises the following steps of: dissolving an artemisinin medicine and an oral iron supplementing agent in an organic solvent to obtain a solution I; dispersing lactoferrin in an inorganic solvent to obtain a solution II; and adding the solution I into the solution II, and uniformly mixing under ultrasonic waves to obtain the ferroptosis-inducing nano-composite. The nano-composite prepared in the invention is loaded with the artemisinin medicine and the oral iron supplementation agent, has the characteristics of simple components and small toxic and side effects and can induce ferroptosis of tumor cells, the anti-tumor effect of the nano-composite is enhanced by regulating macrophages to be in an anti-tumor phenotype through cooperation with innate immunity, and the nano-composite can be used in the field of anti-tumor research.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a ferroptosis-inducing nanocomposite, its preparation method and its application in tumor treatment. Background technique [0002] Ferroptosis is a new type of regulated cell death, and its main features are iron-dependent reactive oxygen species accumulation and lipid peroxidative cell death. As a new target for tumor therapy, ferroptosis is a promising treatment for malignant tumors that cannot be treated by traditional therapies and has shown good therapeutic effects. At present, the development of ferroptosis inducers mainly focuses on the use of iron-based materials to increase the content of ferrous iron in cells and inhibit the enzyme activity of antioxidant system in cells. However, studies have shown that ferroptosis also involves a variety of genes and metabolic pathways, and ferroptosis depends on autophagy. Autophagy can promote ferroptosis by selective...

Claims

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Application Information

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IPC IPC(8): A61K31/357A61K31/194A61K31/721A61K33/26A61K47/42B82Y40/00B82Y5/00A61P35/00
CPCA61K31/357A61K31/194A61K31/721A61K33/26A61K47/42B82Y40/00B82Y5/00A61P35/00A61K2300/00
Inventor 文烈伟梁凌瑕陆翠霞
Owner GUANGXI UNIV
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