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Drug rash plasma exosome circRNA marker and detection primer kit thereof

A technology for detecting primers and markers, used in DNA/RNA fragments, recombinant DNA technology, determination/inspection of microorganisms, etc.

Pending Publication Date: 2021-06-08
AIR FORCE MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CircRNA has no 3' and 5' ends, but has a large number of miRNA binding sites

Method used

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Examples

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Embodiment 1

[0014] Example 1: A drug eruption plasma exosome circRNA marker, including primers and probes for drug eruption plasma exosome long RNA markers and miRNA marker combinations, the long RNA markers include circ_0047921, hsa_circ_0006773 or circ_0056285 One or more, the miRNA markers include one or more of miR-375-3p, miR-200a-3p, miR-122-5p, miR-27a-3p.

[0015] Specifically, the marker is a combination of circ_0047921, hsa_circ_0006773 and miR-375-3p.

[0016] Specifically, the marker is a combination of circ_0047921, miR-200a-3p and miR-122-5p.

[0017] Specifically, the marker is a combination of circ_0047921, hsa_circ_0006773, miR-375-3p, miR-200a-3p and miR-122-5p.

[0018] Specifically, the marker is a combination of circ_0056285, hsa_circ_0006773, miR-27a-3p, miR-375-3p and miR-122-5p.

[0019] A detection primer kit for drug eruption plasma exosome circRNA markers, which also includes reagents required for reverse transcription reaction, reagents required for fluoresce...

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Abstract

The invention discloses a drug rash plasma exosome circRNA marker. The drug rash plasma exosome circRNA marker comprises a primer and a probe of a combination of a drug rash plasma exosome long RNA marker and a miRNA marker; the long RNA marker comprises one or more of circ_0047921, hsa_circ_0006773 or circ_0056285; and the miRNA marker comprises one or more of miR-375-3p, miR-200a-3p, miR-122-5p and miR-27a-3p. The invention provides the drug rash plasma exosome circRNA marker; after clinical verification, the drug rash plasma exosome circRNA marker has relatively high specificity and sensitive value, and has relatively high diagnostic reference value; by analyzing the expression condition of the circRNA in the plasma exosome, whether a subject suffers from drug rash or not can be accurately judged with a large probability; a basis is provided for early discovery of diseases; opportunities are provided for early treatment of patients; the diagnosis accuracy is improved; the diagnosis process is simplified, and the invention has a large application prospect.

Description

technical field [0001] The invention belongs to the technical field of circRNA markers, and more specifically relates to a drug eruption plasma exosome circRNA marker and a detection primer kit thereof. Background technique [0002] Circular RNAs (circRNAs) are a novel class of noncoding RNAs that form covalently closed continuous loops. CircRNAs do not have 3' and 5' ends, but have a large number of miRNA binding sites. Most circRNAs are exonic circRNAs, which are derived from exonic regions of known protein-coding genes by back-splicing. [0003] CircRNA is abundant and stable in exosomes and plasma, which provides a more convenient method for detection. Exosomes are nanoscale double-layered membrane vesicles with a diameter of 30-100 nm, usually in a cup shape. A variety of human cells can release exosomes through extracellular secretion, and these vesicles are widely distributed in blood, saliva, urine and other body fluids. [0004] Drug eruption refers to the adver...

Claims

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Application Information

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IPC IPC(8): C12Q1/6876C12N15/113C12N15/11
CPCC12Q1/6876C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 付萌张晨乔洪江党二乐
Owner AIR FORCE MEDICAL UNIV
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