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Colloidal gold test strip for detecting human parathyroid hormone

A technology of parathyroid hormone and test strips, applied in the field of biomedical detection, can solve problems such as troublesome operation and long time consumption, and achieve the effects of ensuring activity, reducing morbidity, and shortening detection time

Active Publication Date: 2021-06-01
德林医疗科技(武汉)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of venous blood and the processing of serum require the participation of multiple departments, and the operation is cumbersome and time-consuming

Method used

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  • Colloidal gold test strip for detecting human parathyroid hormone
  • Colloidal gold test strip for detecting human parathyroid hormone
  • Colloidal gold test strip for detecting human parathyroid hormone

Examples

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Embodiment Construction

[0026]The principles and features of the present invention will be described below, and the exemplary examples are intended to be construed as they are not intended to limit the scope of the invention.

[0027]1. Screening and preparation of antibodies

[0028]Using parathyroid hormone full protein is used for immune alpaca, preparing antibody antibodies. Using 250 μg of antigen with 250 μl of Frego complete adjuvant, the mixture is used for immune alpaca, and the initial immunization is completed. After 2 weeks and 4 weeks of initiation, the alpaca was enhanced, and the immunization was enhanced using a mixture of 1250 μg of antigen and 250 μl Frego incomplete adjuvant. Two free and three free one week, blood collection was determined by blood collection.

[0029]The results show that the antigenic seconds and three free resistance caused by the anti-blood test value of 3.28 × 10 respectively6And 7.37 × 106. It can be seen that antigen-induced sheep gum produces high titer anti-precision ag...

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Abstract

The invention relates to a test strip for detecting human parathyroid hormone, the test strip comprises a bottom plate, and a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and a water absorption pad which are arranged on the bottom plate and connected in sequence, and a detection line is arranged on the nitrocellulose membrane; the colloidal gold combination pad contains colloidal gold particles labeled by a binding antibody; a capture antibody is anchored in the detection line, both the binding antibody and the capture antibody can specifically recognize and bind parathyroid hormone, and binding sites of the binding antibody and the capture antibody and the parathyroid hormone do not interfere with each other. The colloidal gold test strip disclosed by the invention is convenient and rapid to use, and the detection time is greatly shortened, so that a doctor is helped to judge whether the parathyroid gland is cut by mistake or not in a short time, the activity of the parathyroid gland is greatly ensured, and the incidence rate of parathyroid gland hypofunction after the thyroid surgery is greatly reduced. In addition, the colloidal gold can also be used for detecting a whole blood sample, and can be used for diagnosing parathyroid carcinoma.

Description

Technical field [0001] The invention belongs to the technical field of biomedical detection, and specifically relates to a colloidal gold test strip for detecting human parathyroid hormone. Background technique [0002] The human body has two pairs of parathyroid glands, which are brown in color and shaped like soybeans. They are located in the middle and lower parts of the back (or buried in) of the left and right thyroid lobes respectively. Its main function is to secrete parathyroid hormone (PTH for short) and regulate the metabolism of calcium and phosphorus in the body. The main diseases related to parathyroid glands are hypoparathyroidism complicated by thyroid surgery and primary or secondary parathyroid tumors. [0003] Thyroid cancer is a common malignant tumor that occurs in the head and neck. It is also one of the malignant tumors with the fastest growing incidence rate in recent years. Thyroid cancer has become the fifth most common malignant tumor in women. Th...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/58G01N33/558
CPCG01N33/558G01N33/587G01N33/74G01N2333/635
Inventor 李兴睿
Owner 德林医疗科技(武汉)有限公司
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