Soybean milk ripeness degree rapid detection card and detection method thereof

Abstract

The invention discloses a soybean milk ripeness degree rapid detection card and a detection method thereof, and relates to the field of soybean milk ripeness degree detection. The soybean milk ripeness degree rapid detection card comprises a bottom plate, wherein a sample pad, a reagent pad, a color development pad and a water absorption pad are sequentially pasted on the bottom plate from left to right, the sample pad is made of polyester fibers, the sample pad is soaked in a phosphate buffer solution and dried for later use, the reagent pad is impregnated with a urea solution and dried for later use, and cresol red with a mass percent of 0.5% is dipped on the color development pad. According to the invention, the soybean milk ripeness degree rapid detection card has filtering and impurity removing functions, wherein pigments and small particles in soybean milk liquid to be detected can be filtered and adsorbed, so that the covering effect of the background color of the soybean milk on the water absorbing paper is reduced, and the soybean milk ripeness degree rapid detection card is suitable for pure soybean milk, five-cereal soybean milk, black soybean milk and other soybean milk with various colors in the market added with red dates, Chinese wolfberry fruits and the like; and the method is easy to operate, high in sensitivity, applicable in technology, low in cost, safe to operate, free of flammable and explosive solutions, non-toxic, harmless and free of organic reagents.

Description

technical field [0001] The invention relates to the field of detection of the degree of ripeness of soybean milk, in particular to a quick measurement card for the degree of maturity of soybean milk and a detection method thereof. Background technique [0002] In recent years, food poisoning incidents caused by eating undercooked soybean milk have emerged in an endless stream. Raw soybean milk contains a variety of anti-nutritional factors, such as urease, protease inhibitors, saponins, etc. Excessive consumption will cause a series of adverse reactions, such as, Saponins can strongly stimulate the gastrointestinal mucosa, causing hemorrhagic inflammation and poisoning symptoms. Protease inhibitors will reduce the ability of gastric juice to digest protease, which may cause enlargement and hyperplasia of the pancreas. Urease will catalyze nitrogen-containing substances in the gastrointestinal tract under certain conditions. Decomposition produces ammonia, leading to ammonia ...

AI Technical Summary

Problems solved by technology

However, most of the existing urease activity detection methods are laboratory tests, and the inspection time is long, resulting in distorted results. Therefore, a fast and accurate urease on-site detection method is needed.

[0004] At present, the methods for detecting urease mainly include the national standard Nessler reagent method, the rapid identification method of pH meter, the foam test method and the rapid test box method, etc. Fast, but susceptible to external interference, the error is sometimes slightly larger; Nessler's reagent identification method is reliable, but the steps are cumbersome, and it is not easy to be used for on-site detection; in the foam verification method, the presence and amount of foam vary from person to person. Strict standards can easily cause errors; the rapid test kit can not only quickly and accurately perform qualitative detection of saponins in raw soy milk, but also has clear evaluation standards to avoid external interference, and can meet the requirements of rapid detection of soy milk rawness. However, the reagent contains acetic anhydride and concentrated sulfuric acid, which is corrosive and lachrymatory, and has a low operating safety factor. Therefore, it is urgent to develop a simple, accurate and safe rapid test card for detecting the degree of ripeness of soybean milk.

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