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Labeling reagent and labeling method for schizopygopsis younghusbandi

A technology of labeling reagents and labeling methods, applied in chemical instruments and methods, fish farming, luminescent materials, etc., to achieve the effect of small damage, no toxic side effects, and reduce the impact of fry

Pending Publication Date: 2021-05-28
西藏自治区农牧科学院水产科学研究所
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the fluorescent labeling of the Lhasa naked schizophrenia

Method used

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  • Labeling reagent and labeling method for schizopygopsis younghusbandi
  • Labeling reagent and labeling method for schizopygopsis younghusbandi
  • Labeling reagent and labeling method for schizopygopsis younghusbandi

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Embodiment

[0028] Preparation of cactus extract: wash the fresh cactus with deionized water, mix the fresh cactus and water at a mass ratio of 1:10, and then extract it ultrasonically. The extraction temperature is 50°C, and the extraction time is 60 minutes; the extraction frequency is 75KHz, and the ultrasonic output power is 100W. ; After cooling, filter and centrifuge at 5000r / min to get the supernatant.

[0029] Prepare the labeling reagent for Lhasa naked schistos: the mass ratio of Alizarin Red-S and n-butylbenzisothiazolone is 1:5:1, the amount of cactus extract is 100mL per gram of fluorescent labeling mixture, mix Prepared labeling reagents.

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Abstract

The invention creatively provides a labeling reagent and a labeling method for schizopygopsis younghusbandi. The labeling reagent comprises a fluorescence labeling mixture and a cactus extract, wherein the fluorescence labeling mixture comprises a fluorescence marker, n-butylbenzisothiazolone and vitamin K3, wherein a mass ratio of the fluorescence marker to the n-butylbenzisothiazolone to the vitamin K3 in the fluorescence labeling mixture is 1: (5-10): (1-3), and the addition amount of the cactus extract is that 30-100 mL of the cactus extract is added per gram of the fluorescence labeling mixture. The labeling reagent and the labeling method for schizopygopsis younghusbandi provided by the invention have the advantages that the damage to schizopygopsis younghusbandi is small, and toxic and side effects are avoided; a label formed on the otolith of schizopygopsis younghusbandi has good stability, is not influenced by the outside and can be stored permanently; and large-scale releasing labeling activities can be achieved through one-time marking, and the influence of manual operation on fish fries is reduced to the maximum extent.

Description

technical field [0001] The invention belongs to the technical field of releasing marks, and in particular relates to a marking reagent and a marking method of Lhasa naked schizos. Background technique [0002] Schizopygopsis younghusbandi Regan belongs to Cypriniformes, Cyprnidae, Schizothoracinae subfamily Schizopygopsis, and is distributed in the upper and middle reaches of the Yarlung Zangbo River. , is a unique plateau fish in China (Tibet Autonomous Region Fisheries Bureau, 1995). At present, the researches on the Lhasa scutellaria mainly focus on feeding habits (Yang Xuefeng, 2011), early development (Xu Jing, 2011), individual biology and population dynamics (Duan Youjian, 2015). In recent years, due to overfishing, the resources of Lhasa nudibranch fish have been greatly affected in some areas (Duan Youjian, 2015). In addition, the unscientific release habits have led to the invasion of alien species, resulting in changes in the survival pressure of plateau fish su...

Claims

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Application Information

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IPC IPC(8): C09K11/06A01K61/95
CPCC09K11/06A01K61/95C09K2211/1011Y02A40/81
Inventor 曾本和刘海平王晓明刘飞
Owner 西藏自治区农牧科学院水产科学研究所
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