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Method for solubilizing hydrophobic polyphenol with hydrophilic polyphenols

A hydrophilic and hydrophobic technology, applied in the direction of pharmaceutical formulations, organic active ingredients, food ingredients, etc., can solve problems such as difficult large-scale preparation, difficult control of product quality, immature large-scale preparation technology, etc., to achieve a solution It is difficult to control the quality, improve the water solubility of HPs, solubilize and protect the effect of HPs

Pending Publication Date: 2021-05-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical grafting methods mainly include free radical induction method, chemical coupling method and polyphenol oxidase catalysis method; the two methods of free radical induction and chemical coupling have fast reaction speed, but there are many reaction sites and by-products, and the product quality is difficult to control; The polyphenol oxidase catalytic method has a specific reaction site and few by-products, but the reaction rate is extremely slow, making it difficult to achieve large-scale preparation, and the water-soluble polyphenols are oxidized, resulting in a decrease in antioxidant activity
The difficulty in quality control of polyphenol composite carriers and the immature technology of large-scale preparation limit its practical application

Method used

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  • Method for solubilizing hydrophobic polyphenol with hydrophilic polyphenols
  • Method for solubilizing hydrophobic polyphenol with hydrophilic polyphenols
  • Method for solubilizing hydrophobic polyphenol with hydrophilic polyphenols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 1g of epigallocatechin gallate (EGCG) with a purity of 98% and place it in a 2L container, add 800mL of pure water, stir well, heat up to 35°C, continue stirring until EGCG dissolves, add pure water and make up to 1L , made EGCG solution.

[0034] Weigh 50 mg of curcumin with a purity of 98% and place it in a 50 mL container, add 18 mL of pure ethanol and stir to dissolve, and set the volume to 20 mL to prepare a curcumin solution.

[0035] The above-mentioned 20 mL of curcumin solution was slowly added to 1 L of EGCG solution, and at the same time, the solution was stirred at 200 rpm for uniform mixing, and allowed to stand for 10 minutes to obtain a stable curcumin aqueous solution.

[0036] Pure water was used as a control, that is, an ethanol solution (20 mL) containing 50 mg of curcumin was added to 1 L of pure water, stirred uniformly, and then allowed to stand for 10 minutes.

Embodiment 2

[0038] Weigh 2.5g of bayberry leaf proanthocyanidins with a purity of 85% and place it in a 2L container, add 800mL of pure water, stir evenly, heat up to 35°C, continue to stir until dissolved, filter to remove insoluble impurities, add pure water to make up to 1L, A solution of bayberry leaf proanthocyanidins was prepared.

[0039] Weigh 100 mg of hesperidin with a purity of 98% and place it in a 50 mL container, add 8 mL of dimethylformamide, stir to dissolve, and set the volume to 10 mL to prepare a hesperidin solution.

[0040] The above-mentioned 10 mL of hesperidin solution was slowly added to 1 L of the aqueous solution of bayberry leaf proanthocyanidins, and at the same time stirred at 300 rpm for uniform mixing, and allowed to stand for 10 minutes to obtain a stable aqueous solution of hesperidin.

[0041] Pure water was used as a control, that is, a dimethylformamide solution (10 mL) containing 100 mg of hesperidin was added to 1 L of pure water, stirred uniformly, ...

Embodiment 3

[0043] Weigh 2.0g of tea polyphenols with a purity of 95% and place it in a 2L container, add 800mL of pure water, stir evenly, heat up to 35°C, continue to stir until dissolved, filter to remove insoluble impurities, add pure water to make up to 1L, and make Tea polyphenol solution.

[0044] Weigh 200 mg of 95% turmeric extract in a 50 mL container, add 18 mL of pure ethanol, stir to dissolve, filter to remove insoluble impurities, and set the volume to 20 mL to prepare a turmeric extract solution.

[0045] Slowly add 20 mL of turmeric extract solution to 1 L of tea polyphenol solution, stir and mix at 200 rpm at the same time, and let stand for 10 minutes to obtain a stable aqueous solution of turmeric extract.

[0046] Pure water was used as a control, that is, an ethanol solution (20 mL) containing 200 mg of curcumin extract was added to 1 L of pure water, stirred uniformly, and then allowed to stand for 10 minutes.

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Abstract

The invention discloses a method for solubilizing hydrophobic polyphenol with hydrophilic polyphenol. The method comprises the following steps: 1) dissolving the hydrophilic polyphenol in pure water to prepare a hydrophilic polyphenol aqueous solution; 2) optionally selecting one of the following modes: in the first mode, dissolving hydrophobic polyphenol in an organic solvent to prepare an HPs solution; adding the HPs solution into the hydrophilic polyphenol aqueous solution, and uniformly mixing to obtain an HPs aqueous solution; in the second mode, directly adding hydrophobic polyphenol into the hydrophilic polyphenol aqueous solution, uniform stirring and filtering, and obtaining the HPs aqueous solution. According to the invention, the hydrophilic polyphenol is used as a solubilizing factor, and the hydrophilic polyphenol aqueous solution with a certain concentration and the HPs are simply mixed so that the effects of solubilizing and protecting the HPs can be achieved, the complex preparation of a composite carrier is not needed, and the establishment of an HPs solubilizing system is greatly simplified.

Description

technical field [0001] The invention belongs to the technical field of food biotechnology, and in particular relates to a method for improving the water solubility of hydrophobic polyphenols by utilizing hydrophilic polyphenols. Background technique [0002] Many hydrophobic polyphenols (HPs) have been widely used in the development and production of functional foods due to their unique physiological effects, such as the vascular toughness-enhancing effect of hesperidin and the anti-tumor effect of curcumin. However, HPs has poor water solubility and is sensitive to factors such as oxygen, heat, pH, etc., which makes it difficult to digest and absorb in the human body, easy to degrade during processing and storage, and low in human bioavailability, which seriously affects its health efficacy. [0003] Delivery systems using food-derived components (proteins, polysaccharides, lipids, etc.) as carriers are often used to embed HPs, which not only play a role in solubilization, ...

Claims

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Application Information

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IPC IPC(8): A23L33/105A23L2/52A61K47/22A61K47/26A61K31/12A61K31/7048A61P9/14A61P35/00
CPCA23L33/105A23L2/52A61K47/22A61K47/26A61K31/12A61K31/7048A61P9/14A61P35/00A23V2002/00A23V2200/30A23V2250/082A23V2250/2132A23V2250/2112A23V2250/21164A23V2250/214A23V2250/21166A23V2250/21
Inventor 叶兴乾潘海波陈士国程焕陈健乐徐新雷钱子琪
Owner ZHEJIANG UNIV
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