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Preparation method and application of anticoagulant pig blood and pig plasma for thrombus test

A technology for pig plasma and pig blood is applied in the field of pig plasma preparation and anticoagulation pig blood, which can solve the problems of high price, difficulty in thrombus test, lack of human blood, etc., and achieve the effect of reducing test cost and saving human blood resources.

Inactive Publication Date: 2021-05-28
广州鸿泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the lack of human blood, high price, high biosafety risk and strict supervision, it is difficult to conduct thrombus tests and the cost is high, which seriously limits the development of thromboelastography and coagulation instruments, performance testing and the development of some clinical drugs.

Method used

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  • Preparation method and application of anticoagulant pig blood and pig plasma for thrombus test
  • Preparation method and application of anticoagulant pig blood and pig plasma for thrombus test
  • Preparation method and application of anticoagulant pig blood and pig plasma for thrombus test

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preparation example Construction

[0045] The invention provides a method for preparing anticoagulated pig blood used in a thrombus test, comprising:

[0046] S1, collecting pig blood in a sterile environment;

[0047] S2, add the anticoagulant to the pig blood, shake well and let it stand for 2-4 hours; the anticoagulant plays an anticoagulant effect, and shake it in time after blood collection to make the anticoagulant fully contact with the whole blood;

[0048] S3, after shaking again, aliquoting to obtain anticoagulated pig blood;

[0049] In S2, the anticoagulant is sodium citrate solution, the concentration of the sodium citrate solution is 3.2%-3.8%, and the sodium citrate solution and pig blood are mixed at a weight ratio of 1:9. Sodium citrate inactivates calcium and prevents blood clotting.

[0050]In the present invention, the preparation of anticoagulated pig blood needs to be more rigorous than the preparation of other existing blood. The anticoagulant can only use sodium citrate solution, and t...

Embodiment 1

[0080] S1, collecting pig blood in a sterile environment;

[0081] S2, adding the anticoagulant to the pig blood, shaking well and standing for 3 hours;

[0082] S3, after shaking again, aliquoting to obtain anticoagulated pig blood;

[0083] In S2, the anticoagulant is sodium citrate solution, the concentration of the sodium citrate solution is 3.8%, and the sodium citrate solution and pig blood are mixed at a weight ratio of 1:9.

[0084] The hematocrit of the obtained anticoagulated pig blood is in the range of 29%-35%, and the total protein is in the range of 55g / L-65g / L; the prepared anticoagulated pig blood is placed in an environment of 2-8°C Storage, the validity period is 30 days; within the validity period, the anticoagulated pig blood is used for thrombus test.

[0085] The anticoagulated pig blood prepared in Example 1 was observed, and it was observed that the red blood cells inside were complete, such as Figure 8 As shown, it shows that the anticoagulated pig...

Embodiment 2

[0087] S1, collecting pig blood in a sterile environment;

[0088] S2, adding the anticoagulant to the pig blood, shaking well and standing for 2 hours;

[0089] S3, after shaking again, aliquoting to obtain anticoagulated pig blood;

[0090] In S2, the anticoagulant is sodium citrate solution, the concentration of the sodium citrate solution is 3.2%, and the sodium citrate solution and pig blood are mixed at a weight ratio of 1:9.

[0091] The hematocrit of the obtained anticoagulated pig blood is in the range of 29%-35%, and the total protein is in the range of 55g / L-65g / L; the prepared anticoagulated pig blood is placed in an environment of 2-8°C Storage, the validity period is 30 days; within the validity period, the anticoagulated pig blood is used for thrombus test.

[0092] The anticoagulated pig blood prepared in Example 1 was observed, and it was observed that the red blood cells inside were complete, such as Figure 9 As shown, it shows that the anticoagulated pig...

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Abstract

The invention relates to the technical field of experimental medicaments, and discloses a preparation method and application of anticoagulant pig blood and pig plasma for a thrombus test; the anticoagulant pig blood and the anticoagulant platelet-rich pig plasma prepared by the invention can replace human blood to carry out the thrombus test and simulate human bleeding and hemostasis conditions, so that the thrombus test is successfully carried out, the test cost is reduced, human blood resources can be saved, and meanwhile instrument research and development and performance testing of a thrombelastogram and a blood clotting instrument and development of some clinical drugs are facilitated.

Description

technical field [0001] The invention belongs to the technical field of experimental medicaments, in particular to a preparation method and application of anticoagulated pig blood and pig plasma used in thrombus tests. Background technique [0002] Due to the research and development and preparation of thromboelastography and coagulation instruments, as well as the development of clinical drugs for diseases (such as: stroke, epilepsy, myocardial infarction, uremia, etc.), it is necessary to use anticoagulant and anticoagulant plasma. Thrombosis test. [0003] Among them, thromboelastography is used to monitor the coagulation state of blood samples to assist clinical evaluation of patients. Thromboelastography is widely used in the monitoring of postoperative hemorrhage and (or) thrombus formation during or after cardiac surgery, organ transplantation, tumor and radiotherapy, and assists clinicians in coagulation of clinical patients by monitoring and analyzing the coagulatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02G01N1/28G01N33/86
CPCA01N1/0205A01N1/0221G01N1/28G01N33/86
Inventor 杨夏
Owner 广州鸿泉生物科技有限公司
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