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Lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia

A technology of Lactobacillus plantarum, hyperlipidemia, applied in the direction of lactobacillus, cardiovascular system diseases, function of food ingredients, etc., can solve the problems of large side effects and high cost

Active Publication Date: 2021-05-18
青岛海华莱康生物医药技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drug therapy is the main way to treat hyperlipidemia at present, but the cost of drug therapy is relatively high, and it has relatively large side effects

Method used

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  • Lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia
  • Lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia
  • Lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Isolation and Identification of Lactobacillus plantarum HH19058

[0027] 1. Separation, purification and morphological identification

[0028] Pickle juice: In September 2018, cabbage pickles were collected from farmers in Qingdao, Shandong, and the pickle juice obtained by homogenizing the leaves and juice was diluted 10 times in a gradient.

[0029] Use MRS solid medium, LBS solid medium and NPNL solid medium to isolate the lactic acid bacteria in the above pickle juice, culture the isolated lactic acid bacteria in an anaerobic workstation at 37°C for 48 hours, pick typical colonies on the plate and streak to separate them to obtain pure Colonies were observed morphologically by Gram staining, and cultured anaerobically in MRS liquid medium at 37°C for 24h, and refrigerated at 4°C for later use.

[0030] The result is as figure 1 and figure 2 As shown, the purified strain HH19058 formed a single colony in the MRS medium, and the colonies were almost unif...

Embodiment 2

[0056] Example 2 The Research of Lowering Cholesterol and Triglycerides by Lactobacillus plantarum HH19058

[0057] Inoculate Lactobacillus plantarum HH19058 into cholesterol medium (MRS-TC) at 37°C for 24 hours at 3% inoculum, take 0.2 mL of the culture solution, add 4.8 mL of absolute ethanol and shake and mix for 1 min, then shake and mix after standing for 5 min. Evenly, centrifuge at 4000r / min for 10min, take 2.0mL supernatant for the determination of cholesterol, and use uninoculated cholesterol medium as a control.

[0058] Lactobacillus plantarum HH19058 was inoculated into triglyceride medium (MRS-TG) at 3% inoculum size and cultured at 37°C for 24 hours, then centrifuged at 4000r / min for 10min, and 2.0mL of supernatant was used for the determination of triglyceride. Inoculated triglyceride medium served as a control.

[0059] Cholesterol medium (MRS-TC) preparation: 0.1g of cholesterol, 1.0mL of Tween-20, 0.1g of sucrose ester, 5mL of glacial acetic acid, sonicated ...

Embodiment 3

[0062] Example 3 Effect of Lactobacillus plantarum HH19058 on mice with hyperlipidemia

[0063] Select 108 qualified C57 adult mice, 13 weeks old, half male and half male, the difference in body weight of the same sex should be within 20% of the average body weight, and after adaptive feeding with basal feed for 1 week, according to the average body weight of each group Significant differences were classified into 3 groups (Table 2). Group 1 was fed with normal basal diet, and groups 2 and 3 were fed with high-fat diet, and a mouse model of hyperlipidemia was established by feeding high-fat diet for 4 weeks.

[0064] Table 2 Animal Modeling Grouping

[0065]

[0066] After the model was established, the mice were gavaged at a fixed time every morning according to the treatment method in Table 2 for 4 consecutive weeks, at a rate of 0.1 mL / 10g body weight. During the experiment period, the body weight was weighed every 7 days, and the gavage volume was adjusted.

[0067] ...

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Abstract

The invention discloses lactobacillus plantarum and application thereof in preparation of medicine or food for relieving diseases caused by hyperlipidemia, and belongs to the technical field of microorganism application. The lactobacillus plantarum is lactobacillus plantarum HH19058, and is preserved in the China Center for Type Culture Collection on December 24, 2020, the preservation number is CCTCC NO: M 2020966, and the address is located in Wuhan University, #299, Bayi Road, Wuchang District, Wuhan City, Hubei Province. The lactobacillus plantarum HH19058 is separated from pickled vegetables, has the effects of reducing the blood fat content of the human body and reducing the cardiovascular disease morbidity, and can be used for preparing the food and the medicine for relieving the hyperlipidemia.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of lactobacillus plantarum and its application in preparing medicine or food for alleviating diseases caused by hyperlipidemia. Background technique [0002] With the improvement of people's living standards, the number of patients with hyperlipidemia is increasing and they are getting younger. They often have metabolic diseases such as obesity, hypertension, diabetes and dyslipidemia. These diseases further cause cardiovascular diseases such as coronary heart disease and cerebrovascular accidents. Diseases seriously threaten people's health. Therefore, reducing weight and fat has become an important means of preventing and treating the above-mentioned diseases, and it has also been paid more and more attention by people. Drug therapy is the main way to treat hyperlipidemia at present, but the cost of drug therapy is relatively high, and it...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/10A61P1/16A61P3/04A61P3/06A61P3/10A61P9/12C12R1/25
CPCC12N1/20A61K35/747A61P1/16A61P3/06A61P3/10A61P9/12A61P3/04A61P1/10A23L33/135A23V2002/00A23V2400/169A23V2200/326A23V2200/3262A23V2200/328A23V2200/30A23V2200/3204
Inventor 李明义刘刚马凤龙崔晓辰夏玉洁陈金凤王宗科杨珍
Owner 青岛海华莱康生物医药技术有限公司
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