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Oryza sativa resistance related gene and application thereof

A rice resistance technology, applied in the fields of plant molecular biology and plant genetic engineering, can solve the problems of no rice black-streaked dwarf disease, few anti-virus genes and few disease-resistance-related genes

Active Publication Date: 2021-05-18
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few virus-resistant genes and disease-resistance-related genes in rice known so far, and there are no reports of rice black-streaked dwarf and southern rice black-streaked dwarf resistance-related genes

Method used

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  • Oryza sativa resistance related gene and application thereof
  • Oryza sativa resistance related gene and application thereof
  • Oryza sativa resistance related gene and application thereof

Examples

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Embodiment Construction

[0029] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. The primers involved in the examples of the present invention are from Nanjing GenScript Biotechnology Co., Ltd.

[0030] 1. Preservation and cultivation of test plants and test viruses

[0031](1) The tested rices are NPB (Nipponbare) and W44 (VANDANA). W44 can be purchased from the International Rice Research Institute (IRRI), and is the background plant of the rice genetic transformation of the present invention. Among them, NPB is a susceptible variety of rice black-streaked dwarf virus and southern rice black-streaked dwarf virus, and W44 is a resistant variety of rice black-streaked dwarf virus and sou...

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Abstract

The invention provides an oryza sativa resistance related gene and an application thereof, and belongs to the field of plant molecular biology and plant genetic engineering. The invention provides an oryza sativa protein and an application of a coding gene of the oryza sativa protein. The resistance of oryza sativa to a rice black-streaked dwarf virus and a southern rice black-streaked dwarf virus is regulated and controlled; a rice variety with enhanced resistance to the rice black-streaked dwarf virus and the southern rice black-streaked dwarf virus is bred; the amino acid sequence of the protein is shown in SEQ ID NO.1; and the sequence of the coding gene is as shown in SEQ ID NO. 2. The OsAP47 gene negatively regulates the resistance of oryza sativa to the rice black-streaked dwarf virus and the southern rice black-streaked dwarf virus, can be applied to improvement of crop breeding disease resistance, and is expected to improve the disease resistance of plants to the rice black-streaked dwarf virus and the southern rice black-streaked dwarf virus, so that the purposes of increasing yield and reducing pesticide are achieved.

Description

technical field [0001] The invention belongs to the fields of plant molecular biology and plant genetic engineering, and specifically relates to a rice resistance-related gene and application thereof. Background technique [0002] Rice (Oryza sativa L.) is one of the most important food crops in the world, and it is also one of the most important cultivated crops in my country, but it suffers from severe pests and diseases every year, which brings huge losses to agricultural production. . [0003] Rice black-streaked dwarf disease is a vicious viral disease transmitted by persistent non-ovipositous means by the vector SBPH, which can infect rice, corn and wheat, causing rice black-streaked dwarf disease, corn rough dwarf disease and Wheat green dwarf disease. Rice black-streaked dwarf virus (RBSDV) infection causes severe plant growth abnormalities, resulting in plant dwarfing, failure to normal heading and fruiting, and loss of up to 50% after the onset. Once the plants a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/8283
Inventor 王招云周彤刘斌赵均良孙枫周炼周益军范永坚
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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