Method for accelerating rice generation progress and application

A process, rice technology, applied in the field of plant tissue culture technology and biology, can solve the problems of long plant cycle and complex process

Inactive Publication Date: 2021-05-18
CROPEDIT BIOTECHNOLOGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing literature or patents, it is through the process of callus induction and differentiation into seedlings that the immature embryos are rescued into seedlings. The process is complicated, and the period from embryo rescue to plant acquisition is long, usually about two months.

Method used

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  • Method for accelerating rice generation progress and application
  • Method for accelerating rice generation progress and application
  • Method for accelerating rice generation progress and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Seed preparation: Collect the young ears of Huanghuazhan plants 8, 10, 11, 12, 13, and 14 days after pollination, select the seeds with no mildew on the surface of the seed shell, and carefully peel off the glume.

[0020] (2) Seed disinfection: put the shelled seeds in a 50 mL sterile centrifuge tube. First wash upside down twice with sterile water, then add 75% alcohol to immerse the seeds, shake gently for 10 seconds, and pour off the alcohol. In the ultra-clean bench, add 1.5% sodium hypochlorite solution and disinfect for 5 minutes. Gently invert the centrifuge tube during this time. Remove the sodium hypochlorite solution and wash 5 times with sterile water. Pour the washed seeds into a sterile Petri dish. The sterilized immature rice seeds are as attached figure 1 shown.

[0021] (3) Inoculate the seeds on the seed germination medium, inoculate 1-10 seeds per bottle.

[0022] The formula of the germination medium is: 1 / 2MS medium containing 15g / l of glu...

Embodiment 2

[0026] (1) Seed preparation: Collect the young panicles 15 days after pollination of the gene-edited plants of the rice variety Huanghuazhan, select the seeds with no mildew on the surface of the seed shell, and carefully peel off the glume.

[0027] (2) Seed disinfection: put the shelled seeds in a 50 mL sterile centrifuge tube. First wash upside down twice with sterile water, then add 75% alcohol to immerse the seeds, shake gently for about 10 seconds, and pour off the alcohol. In the ultra-clean bench, add 30ml of 1.5% sodium hypochlorite solution and disinfect for about 8 minutes. Gently invert the centrifuge tube several times during this time. Remove the sodium hypochlorite solution and wash 8 times with sterile water. Pour the washed seeds into a sterile Petri dish with sterile filter paper until inoculated. The sterilized immature rice seeds are as attached figure 1 shown.

[0028] (3) Inoculate the seeds on the seed germination medium, inoculate 1-15 seeds per bo...

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Abstract

The invention provides a method for accelerating the rice generation progress and application. The method comprises the following steps: (1) collecting rice seeds which are pollinated for more than 7 days and can be immature seeds or mature seeds, peeling off seed shells, sterilizing and disinfecting, inoculating the seeds into a seed germination culture medium, culturing and the like, finally forming seedlings, and transplanting the seedlings into a soil pot or a field. The method can be used for saving seeds of precious rice germplasm, gene editing materials and transgenic materials which are extremely small in seed quantity or cannot be naturally mature due to limitation of growth conditions and seasons, and can also enable the rice seeds to quickly enter the next generation, so that the rice growth cycle is obviously shortened, and the generation progress is effectively accelerated.

Description

technical field [0001] The invention belongs to the field of plant tissue culture technology and biotechnology, and specifically relates to a culture medium, a culture method, an application and the like for cultivating seedlings of rice seed groups. Background technique [0002] In the process of traditional rice breeding and genetic engineering breeding, according to the traditional rice breeding planting method, for most varieties, it takes several months from preparing to sow to harvesting mature and usable seeds. It can also only be grown for two generations. The rice growth cycle is roughly divided into two stages: the vegetative growth stage and the reproductive growth stage. The vegetative growth stage is about 90 days from rice seed germination to seedling jointing stage, and a total of about 35 days from rice seed germination to transplanting. The reproductive growth stage is called the reproductive growth stage from booting to maturity, and it takes about 70 day...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 孟钊红杨敏王金羽
Owner CROPEDIT BIOTECHNOLOGY INC
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