Single cell automatic separation system and method

Abstract

The invention discloses a single cell automatic separation system and method. The system comprises a cell solution container, a cell culture solution container, a cell waste liquid container, a single cell container, a cell separation flow channel and a cell detection device. The cell separation flow channel comprises a bent liquid inlet flow channel I connected with a liquid inlet pipe I, a liquid inlet flow channel II connected with a liquid inlet pipe II, and a main flow channel converging the liquid inlet flow channel I and the liquid inlet flow channel II, the main flow channel is connected with a waste liquid pipe through a branch flow channel I, the main flow channel is connected with a liquid outlet pipe through a branch flow channel II, the liquid inlet pipe I is inserted into the cell solution container, the liquid inlet pipe II is inserted into the cell culture liquid container, and the waste liquid pipe is inserted into the cell waste liquid container; a three-way pipe, an injection valve and a nozzle are sequentially arranged on the liquid outlet pipe behind the liquid outlet valve; and the three-way pipe is connected with one end of the injection pipe, and the other end of the injection pipe is inserted into the cell culture solution container. The system and the method disclosed by the invention are high in single cell separation accuracy, high in separation speed, simple in system structure and low in cost.

Description

technical field [0001] The invention relates to the technical field of cell separation, in particular to a single cell automatic separation system and method. Background technique [0002] Cells contain a large amount of important information in life sciences, and the heterogeneity of cell populations leads to the possibility that cells of the same type may exhibit different characteristics. Therefore, the analysis of cell populations may lose important information about individuals in the larger context. Single-cell analysis has the following advantages: 1) A single cell contains all the genetic information of a species; 2) Single-cell experiments avoid the interference of cell heterogeneity and are more accurate; 3) Single-cell experiments are conducive to restoring biological events themselves, Many biological events, such as embryonic development, tumor formation and metastasis, start from single cells. Single cell analysis is of great significance for drug screening, s...

AI Technical Summary

Problems solved by technology

[0003] Among the traditional single cell separation techniques, the limiting dilution technique (Limiting Dilution) is simple and easy to operate, but the accuracy of single cell separation is low

Fluorescence activated cell sorting (FACS) system has high separation efficiency and high accuracy, but the system structure is complicated and the cost is high

Laser Capture Microdissection (LCM) is often used to precisely separate a single cell from a tissue. This method has high accuracy, but the separation efficiency is low and requires high requirements for ...

Images