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Preparation and application of novel fluorescent probe having ICT-PET effect and capable of being used for formaldehyde detection

An ICT-PET, fluorescent probe technology, applied in the field of biological analysis and detection, chemical analysis, can solve the problems of disrupting the dynamic balance, affecting, consuming the liquid to be tested, etc.

Active Publication Date: 2021-05-14
HUNAN TARGETING DETECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these probes are designed and developed by specific reaction with FA, and usually consume FA in the test fluid or even cells, which may affect or destroy the homeostasis

Method used

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  • Preparation and application of novel fluorescent probe having ICT-PET effect and capable of being used for formaldehyde detection
  • Preparation and application of novel fluorescent probe having ICT-PET effect and capable of being used for formaldehyde detection
  • Preparation and application of novel fluorescent probe having ICT-PET effect and capable of being used for formaldehyde detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Synthesis of compound 1

[0026] A mixture of 2-aminothiophenol (375.57 mg, 3.0 mmol) and 3-aminosalicylic acid (459.39 g, 3.0 mmol) was dissolved in 10 ml of polyphosphoric acid (PPA). Stir at 185°C until the reaction is complete, then pour the reaction system into cold water, and a yellow precipitate occurs. Suction filter, collect filter cake, and use 10%Na 2 CO 3 The filter cake was washed with solution to obtain 622.63 mg of green solid with a yield of 85.7%.

[0027] Synthesis of compound 2

[0028] Compound 1 (484.58 mg, 2 mmol) and maleic anhydride (245.13 mg, 2.5 mmol) were dissolved in 40 ml of glacial acetic acid, and heated to reflux until the reaction was complete. After the reaction system was cooled, it was poured into cold water to obtain a yellow precipitate. Suction filter, collect filter cake, and use 10%Na 2 CO 3 The filter cake was washed with the solution, purified by silica gel column chromatography, and the solvent was removed by rotary eva...

Embodiment 2

[0032] Preparation of fluorescent probe master solution

[0033] Accurately weigh 4.74 mg of the product obtained from the above separation with a purity of more than 99% and carefully transfer it to a 50 mL volumetric flask, add CH at room temperature 3 CN solution was completely dissolved, and the volume was adjusted to the mark to obtain a probe mother solution with a concentration of 1 mM. During the test, 20 μL of the above solution was measured with a microsampler each time, and dissolved in the test system so that the total volume of each test was 2 mL, and the concentration of the fluorescent probe was 10 μM.

Embodiment 3

[0035] Preparation of FA mother liquor

[0036]FA was prepared with PBS buffer solution to prepare 5 mL stock solutions with different concentration gradients (0.1 mM, 0.2 mM, 0.4 mM, 0.7 mM, 1.0 mM, 1.5 mM, 2.0 mM, 3.0 mM). The analytes to be used in the rest of the tests were respectively prepared with PBS buffer solution into a stock solution with a concentration of 3 mM.

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Abstract

The invention relates to preparation and application of a novel fluorescent probe having an ICT-PET effect and capable of being used for formaldehyde detection, which belong to the technical field of analytical chemistry. The chemical structure of the novel fluorescent probe is shown as a formula (I). The invention also discloses a preparation method of the fluorescent probe and application of the fluorescent probe in detection of environment and biological samples. The fluorescence intensity of the probe is obviously improved along with the increase of the formaldehyde content, and the probe can be used for formaldehyde detection and fluorescence imaging in different systems such as environment and living cells, and has a good application prospect. The formula (I) is as shown in the specification.

Description

[0001] The present invention relates to a fluorescent probe, in particular to a method for preparing a novel fluorescent probe with ICT-PET effect which can be used for formaldehyde detection, and in particular to the application of the fluorescent molecular probe in the detection of formaldehyde in the environment and in vivo, The invention belongs to the technical field of chemical analysis and biological analysis and detection. Background technique [0002] Formaldehyde (Formaldehyde, FA) is a colorless gas with a strong pungent odor. It has sensitization, teratogenic and carcinogenic toxicity to the human body, and is a recognized carcinogenic and teratogenic substance. At the same time, studies have shown that endogenous FA produced by organisms is an important chemical substance in living organisms, providing important carbon units for the physiological and chemical reactions of animals and humans. However, under the influence of pathological and external adverse factor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D417/10G01N21/64
CPCC09K11/06C07D417/10G01N21/6428C09K2211/1029C09K2211/1037C09K2211/1007
Inventor 不公告发明人
Owner HUNAN TARGETING DETECTION TECH CO LTD
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