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Digital microfluidic library construction system

A library construction and digital micro technology, applied in chemical library, combinatorial chemistry, library creation, etc., can solve the problems of insufficient chip reliability and high cost, and achieve low cost, short time consumption, and improved efficiency and accuracy. Effect

Inactive Publication Date: 2021-04-30
张研
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Illumina's Neoprep product was recalled in 2017 due to insufficient chip reliability. The chip of Oxford Nanopore's Voltrax product is manufactured using a thin-film transistor process, so the cost is high, and this product is only suitable for library construction of Oxford Nanopore's own sequencing equipment

Method used

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  • Digital microfluidic library construction system
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  • Digital microfluidic library construction system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1: Library Construction of DNA Samples

[0095] figure 1 Schematic diagram of chip construction for digital microfluidic DNA library, which includes multiple sample / reagent inlets 1, multiple storage and distribution areas 2, waste liquid area 3, product outlet 4, low temperature area 5, variable temperature area 6, magnetic beads Separation point 7.

[0096] The process of using the digital microfluidic DNA library construction chip to construct a DNA library is as follows:

[0097] The sample solution is manually prepared first, and the step of manually preparing the sample solution specifically includes: manually preparing a mixture of the sample, deionized water, and buffer solution, and oscillating to mix evenly. The reason why this step is manual is that the required sample volumes are different for different types of samples.

[0098] Then, fully-closed fully automatic chip-type DNA library construction is carried out, including the following steps: ...

Embodiment 2

[0111] Example 2: Library Construction of RNA Samples

[0112] Figure 4 Schematic diagram of chip construction for digital microfluidic RNA library, including multiple sample / reagent inlets 1, multiple storage and distribution areas 2, waste liquid area 3, product outlet 4, low temperature area 5, variable temperature area 6, magnetic beads Separation point 7.

[0113] The process of using the digital microfluidic RNA library to construct a chip to construct an RNA library is as follows:

[0114] The sample liquid is manually prepared first, and the step of manually preparing the sample liquid specifically includes: manually preparing a mixed liquid of the sample and deionized water, and vibrating to mix evenly. The reason why this step is manual is that the required sample volumes are different for different types of samples.

[0115] Then, carry out the construction of fully enclosed fully automatic chip RNA library, including the following steps:

[0116] (1) Sample lo...

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Abstract

A chip is constructed through a full-flow full-automatic digital micro-fluidic library, full-flow library construction of a sample is included on a single chip, and the chip comprises a low-temperature area used for keeping effectiveness of a reagent in the waiting process; the system comprises: a temperature change zone which is used for providing temperature conditions required by each reaction in library construction; and liquid storage and distribution areas 2 which can be used for respectively distributing washing liquid, magnetic bead solution and eluent drops. A user only needs to load a sample to the chip to realize full-process DNA or RNA library construction.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, and relates to a digital microfluidics library construction system. Background technique [0002] Library construction is one of the necessary steps for DNA and RNA sequencing. The library construction process is relatively complex and usually includes many operations such as fragmentation, end repair, adapter ligation, magnetic bead washing, and library amplification. The steps of the library construction process are cumbersome and time-consuming. The current library construction work mainly relies on manual operations or large workstations based on robotic arms. For example, Beckman Coulter's fully automated laboratory sample processing workstation The 4000 can complete liquid handling tasks such as pipetting, gradient dilution, liquid separation, and liquid merging, freeing researchers from the complicated experimental operations of library construction. Illumina's Neoprep pro...

Claims

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Application Information

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IPC IPC(8): C40B50/00
CPCC40B50/00
Inventor 苏阳张研
Owner 张研
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