Dual fluorescent quantitative PCR primer and kit for Escherichia coli disease and Lyme disease

Abstract

The invention discloses a dual fluorescent quantitative PCR primer and a kit for Escherichia coli disease and Lyme disease. The dual fluorescent quantitative PCR primer for the Ehrlichi disease and the Lyme disease comprises a primer for detecting the Ehrlichi disease and a primer for detecting the borrelia burgdorferi; the kit contains the Ehrlichia body disease and Lyme disease dual fluorescent quantitative PCR primer. The kit disclosed by the invention can be used for simultaneously detecting Ehrlichia and Borrelia borreliae, and has the advantages of rapidness in detection, high efficiency, accuracy in quantification, high sensitivity and strong specificity.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a dual fluorescence quantitative PCR primer and a kit for ehrlichiosis and Lyme disease. Background technique [0002] The multiplex fluorescent PCR method has high detection efficiency, that is, multiplex fluorescent PCR can realize multiple inspections in one tube, simplifying the operation process; saving reagent costs and personnel operating time, especially when carrying out a large number of samples, significantly reducing detection costs and operating time and other advantages . SYBR Green I dye-based fluorescent quantitative PCR is cost-effective, and the linear relationship between the fluorescence signal intensity of SYBR Green dye and the amount of double-stranded DNA can be used to monitor the amount of double-stranded DNA in the PCR system and use the melting curve for identification diagnosis. The melting curve is a curve that reflects the degree of degradatio...

AI Technical Summary

Problems solved by technology

At present, the diagnosis of ehrlichiosis is mainly performed by Giemsa staining of blood cells, and the presence or absence of morula bodies under a microscope. Although there are currently effective national standards for the diagnosis of Lyme disease, the recommended pathogen isolation and serological testing The operation is difficult, the cycle is long, and the requirements for operators are high, and there is currently a lack of methods and standards for diagnosing these two diseases at the same time

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