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Tissue culture method of alstroemeria

A technique of tissue culture and culture medium, applied in the direction of culture medium, horticultural methods, botany equipment and methods, etc., can solve problems such as no successful technical route for Alpinia japonica tissue culture, and achieve fast propagation speed and high emergence rate , The effect of good quality seedlings

Active Publication Date: 2021-04-30
山东和正生态农业开发有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the literature and patent search, there is no technical route for the successful tissue culture of Alpinia miltiorrhiza

Method used

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  • Tissue culture method of alstroemeria
  • Tissue culture method of alstroemeria
  • Tissue culture method of alstroemeria

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Embodiment Construction

[0016] In order to clearly illustrate the technical features of the present solution, the present application will be described in detail below through specific implementation modes.

[0017] The cultivation process of this application comprises the following steps:

[0018] S1. Explant treatment:

[0019] The explant among the present invention is the flower stem part of the fresh-cut flowers of Alpinia japonica; the explant is rinsed with running water for about 30 minutes, and after rinsing, it is washed with a detergent. Wash the intersections of the stems with a brush, and then rinse with clean water for about 1 hour. After rinsing, move to the ultra-clean workbench for explant sterilization; the explants are sterilized with alcohol for 10-15 seconds, and then treated with mercury chloride 3-5min, and finally rinse with sterile water 5-10 times;

[0020] S2. Induction of isolated buds;

[0021] The explants are inoculated on the isolated bud induction medium for cultur...

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Abstract

The invention relates to a tissue culture method of alstroemeria. The tissue culture method comprises the following steps: performing in vitro bud induction, subculture, rooting culture and acclimatization and transplantation on an explant, wherein a culture medium for in vitro bud induction and subculture is an MS culture medium and contains 6-BA and NAA, and the explant is a stem part of a fresh cut flower of the alstroemeria. According to the invention, special selection is performed on explant parts instead of conventional parts such as underground rhizomes, which is a basis of successful tissue culture of the alstroemeria, and the other invention point is selection of the culture medium, especially an in-vitro bud induction culture medium and a subculture culture medium, which are based on an MS culture medium, and the 6-BA and the NAA with concentration gradient coordination are compounded, so that rapid tissue culture of the alstroemeria is realized.

Description

technical field [0001] The present application relates to a method for tissue culture of alpinia officinalis. Background technique [0002] Alternaria, also known as Peruvian lily, belongs to Amaryllidaceae. Alternaria is a perennial herbaceous flower. Because of its rich color, unique shape, elegant and rich bloom, it has become a rookie of cut flowers. In recent years, it has been ranked among the top ten cut flowers in the world. In recent years, it has begun to be used in potted ornamental plants. Due to the monopoly of a small number of large foreign companies, Altium alba is still in the stage of introduction in my country, and it is rare in the cut flower market. It is only sold in a certain amount in the high-end cut flower markets of a few big cities such as Beijing and Shanghai. With the improvement of people's living standards, the national flower consumption is increasing at an average annual rate of more than 10%, and alstroemeria, as a newcomer to cut flowers...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/15A01G24/28
CPCA01H4/008A01H4/001A01G31/00A01G24/15A01G24/28
Inventor 程曼范士猛梁凤龙马志刘会君
Owner 山东和正生态农业开发有限公司
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