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Microbial agent capable of degrading kitchen garbage and application thereof

A technology for microbial inoculants and kitchen waste, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of long degradation treatment time, incomplete fermentation, low degree of reduction, etc. The effect of degradation and adaptability, less environmental pollution, and lower production costs

Active Publication Date: 2021-04-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kitchen waste processor is equipped with a deodorization device, an oil-water separation device, and a crushing and extrusion device, which creates good conditions for the fermentation of the biological warehouse and reduces environmental pollution. It is currently widely used at home and abroad, but , kitchen waste processors still have problems such as long degradation treatment time, low degree of reduction, incomplete fermentation, etc., and screening efficient degrading bacteria agents is an effective way to improve the degradation efficiency of kitchen waste processors

Method used

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  • Microbial agent capable of degrading kitchen garbage and application thereof
  • Microbial agent capable of degrading kitchen garbage and application thereof
  • Microbial agent capable of degrading kitchen garbage and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: the preliminary selection and breeding of composite bacterial agent

[0057] Specific steps are as follows:

[0058] Bacillus subtilis ACCC60364, Bacillus pumilus ACCC04180, Bacillus megaterium ACCC04366, Mesophilic sphingobacterium CICC22013, Bacillus tequila ACCC06525, Bacillus licheniformis ACCC01942 were inoculated on solid starch selection medium, solid protein selection medium, In the solid fat selective medium and solid cellulose selective medium, observe the ratio of the diameter of the transparent circle to the diameter of the colony after culturing in a 45°C incubator for 24 hours. The larger the ratio, the stronger the degradation ability.

[0059] The diameter ratio of each strain is shown in Table 2.

[0060] Table 2 Ratio of transparent circle to bacterial diameter of each bacterial strain on protein, fat, starch, and cellulose plates

[0061]

[0062]

[0063] The ability of the strain to degrade starch, protein, fat and cellulose wa...

Embodiment 2

[0068] Embodiment 2: the preparation of microbial solid bacterial agent

[0069] The specific method is as follows:

[0070] (1) Preparation of kitchen waste leaching solution: crush the kitchen waste with a pulverizer and mix well, put it in a heating container, add 2-4 times the quality of water, heat at 100°C for 1-2h, and pass through a 10-20 mesh sieve after cooling , put the liquid part in a 4750r / min centrifuge at room temperature and centrifuge for 0.5-1h, remove the oil and take the liquid part;

[0071] Preparation of solid kitchen waste leaching liquid culture medium: adding 1.5-2% agar to the kitchen waste leaching liquid;

[0072] (2) Inoculate Bacillus subtilis, Bacillus pumilus, Bacillus tequilensis and Sphingobacterium thalpophilum into solid kitchen waste leachate culture medium respectively Carry out seed pre-cultivation, culture in a constant temperature incubator at 45°C for 8-12 hours, select a single colony with a diameter greater than 1.5 mm and inocul...

Embodiment 3

[0092] Example 3: Determination of COD content in the supernatant of the complex bacterial agent fermentation liquid during the preparation of the kitchen waste leachate

[0093] Inoculate single colonies of Bacillus subtilis, Bacillus pumilus, Bacillus tequila, and mesophilic Sphingobacterium prepared in step (2) of Example 2 into the kitchen waste leachate and culture them for 30 hours to obtain fermentation broths respectively, The supernatant obtained by centrifugation was tested for COD to determine the utilization rate of the compound bacterial agent to the medium during the cultivation process.

[0094] As a control, the seed liquid of different strains activated by LB was also inoculated into the leachate of kitchen waste to obtain fermentation liquid, and the supernatant obtained by centrifugation was used for COD determination.

[0095] The COD value of the raw liquid of the kitchen waste leachate was 20000±5000 mg / L, and the COD content of the effluent obtained from...

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Abstract

The invention discloses a microbial agent capable of degrading kitchen garbage and application of the microbial agent. The microbial agent is prepared from bacillus subtilis, bacillus pumilus, Sphingobacterium thalpophilum and bacillus tequilensis. The four strains are pre-cultured and activated by using a kitchen waste leachate and are prepared into the complex microbial inoculant, so that the degradation efficiency of the kitchen waste can be obviously improved, the degradation rate is improved by 15.5% compared with that of LB culture medium culture preparation, the COD of supernatant effluent after fermentation is completed is less than 200mg. L <-1 >, and the environmental pollution is small; the kitchen garbage leachate is used for pre-culturing and preparing the microbial inoculum, so that the production cost of the solid microbial inoculum can be greatly reduced, the wastewater treatment capacity is reduced, and the application prospect is higher.

Description

technical field [0001] The invention relates to a microbial agent capable of degrading kitchen waste and its application, and belongs to the technical field of kitchen waste treatment. Background technique [0002] The main components of kitchen waste include rice and flour food residues, vegetables, meat and bones, etc., and are often mixed with various impurities such as glass, chopsticks, plastic bags, and cans. It is of great significance to realize the reduction, recycling and harmless treatment of kitchen waste to maintain the sustainable development of our living environment. [0003] At present, domestic and foreign kitchen waste treatment methods are mainly divided into two categories. One is non-biological treatment, which mainly includes sanitary landfill, crushing and direct discharge, incineration, etc. The filtrate is easy to cause secondary pollution to the environment; the level of incinerated resources is extremely low, and it is easy to cause secondary pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/02B09B3/00C12R1/125C12R1/07C12R1/01
CPCC12N1/20C12N11/10C12N11/02B09B3/00
Inventor 诸葛斌陈海燕宗红陆信曜
Owner JIANGNAN UNIV
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