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High-flux multi-species bacterial colony counting method

A counting method and colony-like technology, applied in computing, image data processing, instruments, etc., can solve the problem of low accuracy of high-throughput detection, achieve the effect of solving the problem of low accuracy and improving throughput

Pending Publication Date: 2021-04-20
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] In view of the deficiencies in the above-mentioned technologies, the present invention provides a high-throughput multi-type colony counting method, which is used to solve the problem of low accuracy of high-throughput detection and improve the throughput and counting accuracy of simultaneous detection of culture dishes and efficiency

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  • High-flux multi-species bacterial colony counting method
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  • High-flux multi-species bacterial colony counting method

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Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0025] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0026] Such as Figure 1-3 As shown, the present embodiment provides a high-throughput multi-type colony counting method, comprising the following steps:

[0027] S10, in-situ timing detection: turn on the background light source, aim several cameras at a petri dish to be observed, and perform in-situ timing detection on several petri dishes to be observed at the same time, acquire and save real-time images of all petri dishes;

[0028] S20, colony identification and counting: load real-time images of all petri dishes, and automatically identify and count colonies through image pr...

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Abstract

The invention discloses a high-flux multi-species bacterial colony counting method which comprises the following steps: in-situ timing detection: turning on a background light source, aligning a plurality of cameras with a culture dish to be observed, performing in-situ timing detection on the plurality of culture dishes to be observed at the same time, and acquiring and storing real-time images of all the culture dishes; bacterial colony identification and counting: loading real-time images of all culture dishes, and automatically identifying bacterial colonies and counting through image preprocessing. The method is used for solving the problem of low accuracy of high-flux detection and improving the flux, counting accuracy and efficiency of simultaneous detection of the culture dish.

Description

technical field [0001] The invention relates to the technical field of biological reagent detection, more specifically, the invention relates to a high-throughput multi-type colony counting method. Background technique [0002] Microbial colony counting is a common biological detection method, which is widely used in microbial detection in various environments such as drinking water, food / drugs, clean workshops, cabin surfaces, and pathogenic microorganism laboratories. After the sample is sampled, directly or after appropriate dilution, it is spread on the surface of the solid medium. After a certain incubation time, the number of bacteria in the original sample is judged after the formation of visible colonies. As a gold standard method, the traditional culture method is combined with visual counting, and the result reporting time is 2-5 days, which cannot meet the counting needs in emergency situations. For example, when several microorganisms are very close in physical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06T7/13G06T7/136G06T7/90G06T5/30G06T5/00
Inventor 王磊尹焕才陈名利潘宇骏
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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