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Preparation method of efficient biocontrol growth-promoting ginseng composite microbial fertilizer

A technology of compound microbial bacteria and microorganisms, which is applied in the preparation of compound microbial fertilizers for microbial multi-antibiotic biocontrol, high efficiency, and activated fertilizer efficiency, to achieve the effects of environmental friendliness, low cost, and promotion of absorption

Inactive Publication Date: 2021-04-20
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problem that the existing non-forest land cultivation of ginseng mainly uses chemical fertilizers and pesticides to achieve the purpose of increasing the yield of ginseng, and provides a compound microbial fertilizer to promote the reuse of large amounts of nutrient elements nitrogen, phosphorus and potassium in non-forest land soil. Preparation method of composite microbial bacterial fertilizer for improving ginseng yield and quality through effective absorption of nutrients by ginseng root system, high-efficiency multi-antibiotic biocontrol

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  • Preparation method of efficient biocontrol growth-promoting ginseng composite microbial fertilizer

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specific Embodiment approach 1

[0016] Specific implementation mode one: this implementation mode is a kind of high-efficiency, increase fertilizer efficiency, microbial multi-antibiotic biocontrol ginseng compound microbial bacterial fertilizer preparation method, is completed according to the following steps today:

[0017] 1. Collection: ①. Collection time: collect during the ginseng harvest period in autumn, when the nutrient content of ginseng root system is at a relatively high level; ②. Selection requirements: choose 4-6 year old ginseng that is healthy and healthy, and has no disease. The ginseng rhizosphere soil species with seedling height of 39-47cm, root length greater than 10cm at rest, root width of 1-3cm at rest, and disease-free non-forest ginseng soil; Dig out the fallen leaf layer and the soil around the root system of ginseng seedlings, carefully take out the ginseng root, shake the rhizosphere soil carefully, collect it in a sterile 50ml centrifuge tube and seal it well, and put dry ice ou...

specific Embodiment approach 2

[0027] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is: step 3 ③, the preparation of compound microbial bacterial fertilizer is 0.5% carbon source, 1.5% nitrogen source, fermentation temperature 29 ± 1 ℃, fermentation liquid bottling Under the conditions of 125ml and initial inoculation amount of 2±1%, 65 parts of compound microbial fertilizer were prepared in parts by weight, including 25 parts of Bacillus subtilis, 20 parts of Bacillus licheniformis, 15 parts of marine Bacillus, and Bacillus sp. 5 parts of bacteria; the weight-to-number ratio of Bacillus subtilis, Bacillus licheniformis, marine Bacillus, and bacteria of the genus Bacillus in the prepared composite microbial fertilizer is 5:4:3:1; the others are the same as in Embodiment 1.

specific Embodiment approach 3

[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one is: step three ③, the preparation of compound microbial bacterial fertilizer is at carbon source 0.5%, nitrogen source 1.5%, fermentation temperature 29 ± 1 ℃, fermentation liquid bottling Under the conditions of 125ml and initial inoculation amount of 2±1%, 70 parts of compound microbial fertilizer were prepared in parts by weight, including 25 parts of Bacillus subtilis, 20 parts of Bacillus licheniformis, 15 parts of marine Bacillus, and Bacillus spp. 10 parts of bacteria; the weight-to-number ratio of Bacillus subtilis, Bacillus licheniformis, marine Bacillus, and bacteria of the genus Bacillus in the prepared composite microbial fertilizer is 5:4:3:2; others are the same as in Embodiment 1.

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Abstract

The invention discloses a preparation method of an efficient biocontrol growth-promoting ginseng composite microbial fertilizer, relates to the technical field of ecological planting of ginseng, and solves the problem that the yield of ginseng is increased mainly by chemical fertilizers, pesticides and the like in existing non-forest land ginseng cultivation. The preparation method comprises the following steps: I, collecting; 2, screening and separating; and 3, preparing the compound microbial fertilizer. The method has the advantages that: 1, the four strains have strong stress resistance and multiplication capacity and promote growth mutually; 2, the quantity of viable bacteria is large and can reach 16.67 * 10 < 9 > CFU * ml < -1 >; 3, the fertilizer efficiency is activated, the reutilization of nitrogen, phosphorus and potassium is increased, the absorption of ginseng roots is promoted, the fertilizer has slow release property, persistence and long fertilizer efficiency, and the yield and quality of ginseng are improved; 4, the structural characteristics of soil microbial florae are improved, the diversity of effective microbial florae is increased, and the biocontrol function is achieved; and 5, the method is simple to operate, low in cost, remarkable in effect, environment-friendly and the like. The method is mainly used for ecological planting biocontrol, fertilizer efficiency increase and yield increase of ginseng.

Description

technical field [0001] The invention belongs to the technical field of ecological planting of medicinal plants, and specifically relates to a method for preparing a compound microbial fertilizer with high efficiency, activated fertilizer effect and multi-microbial resistance biocontrol. Background technique [0002] Ginseng (Panax ginseng C. A. Mey.), known as the king of herbs, has attracted much attention because of its outstanding efficacy and good modern pharmacological activity. For a long time, due to over-harvesting and man-made environmental damage, wild ginseng resources are now on the verge of extinction. Due to the scarcity of wild ginseng resources, it is inevitable to cultivate a large number of ginseng artificially. In recent years, with the implementation of national policies such as returning farmland to forests, the deepening of the concept of ecological planting of medicinal plants, and the increasing awareness of ginseng farmers on ginseng land protection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05G3/80C05F17/20C05F17/50C12N1/20C12R1/125C12R1/10C12R1/07C12R1/01
CPCY02W30/40
Inventor 罗云清许永华赵玥卢宝慧董岭刘文丛
Owner JILIN AGRICULTURAL UNIV
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