Amauroderma rugosum strain and cultivation, picking and preservation method thereof
A technique for wrinkling and cultivating false sesame seeds, applied in the field of microorganisms, can solve the problems of uneven product phase, influence of active ingredients, and inability to keep up with production and yield, and achieves the effects of good taste, strong aroma and fast growth rate.
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Embodiment 1
[0055] The cultivation, collection, drying method of embodiment 1 new wrinkled cap pseudo-sedum strain RFC
[0056] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed;
[0057] The formula of the mother seed medium is: 20% of potato, 1.5% of agar, 1% of glucose, 0.2% of fish meal, 0.1% of potassium dihydrogen phosphate, and the balance is water.
[0058] S2 original seed preparation: the original seed culture material with a moisture content of 63% and a pH value of 5.4-5.6 was put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother species into a sterilized bag containing the original seed compost, and cultivate it at 22-28°C until the hyphae are covered with the fungus bag; the original seed...
Embodiment 2
[0064] Cultivation, collection, drying method of embodiment 2 new wrinkled cap false mushroom classification RFC
[0065] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed; mother seed medium formula It is: 25% of potato, 1.3% of agar, 1.5% of glucose, 0.2% of fish meal, 0.1% of potassium dihydrogen phosphate, and the balance is water.
[0066] S2 Preparation of the original seed: The original seed culture material with a pH value of 5.4-5.6 is put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother species into a sterilized bag containing the original seed culture material, and cultivate it at 22-28°C until the hyphae are covered with the bacteria bag; the original seed culture material incl...
Embodiment 3
[0071] Cultivation, collection, drying method of embodiment 3 new wrinkled cap false mushroom classification RFC
[0072] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed; mother seed medium formula For: potato 22%, agar 1.4%, glucose 1.7%, fish meal 0.2%, potassium dihydrogen phosphate 0.1%, the balance is water
[0073] S2 Preparation of the original seed: The original seed culture material with a pH value of 5.4-5.6 is put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother seed into the sterilization bag with the original seed culture material, and cultivate it at 22-28°C until the mycelium is covered with the bacteria bag; the original seed culture material includes 40% bamboo chips, fi...
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