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Amauroderma rugosum strain and cultivation, picking and preservation method thereof

A technique for wrinkling and cultivating false sesame seeds, applied in the field of microorganisms, can solve the problems of uneven product phase, influence of active ingredients, and inability to keep up with production and yield, and achieves the effects of good taste, strong aroma and fast growth rate.

Pending Publication Date: 2021-04-20
福州美利莎生物科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The current method of planting wrinkled-covered false mushrooms requires the use of a large number of branches, leaves and wood of the Taiwan Acacia tree. The Latin scientific name of the Taiwan Acacia tree is Acacia confusa, which belongs to the ecological forest. It is not in line with the national ecological response to use it in large quantities. The actual production output cannot keep up, and the corresponding formula cannot be mass-produced in a factory in terms of raw material output and economic benefits.
Mainly because there is no breakthrough in cultivation technology, the cultivation ingredients and growth environment of the parasitic tree species that are separated from it have not been found, and more suitable strains have not been screened
[0007] 2. The existing technology does not distinguish the drying and storage of the rugosa mushroom from other edible and medicinal fungi, resulting in the fact that the dried mushroom cannot preserve its unique "blood", the active ingredients are greatly affected, and the development potential is also low. Greatly limited and of varying quality
The main reason is that the research on the wrinkled-capped fake sesame is not deep enough, and there is no breakthrough in the drying technology of the wrinkled-covered fake sesame
[0008] 3. The existing fresh wild products of wrinkled-capped false ganoderma are not sold in the market, and there is no red "blood" flowing out after touching or soaking in water, which cannot reflect the "blood ganoderma". Therefore, wild products are all dry products, often called black ganoderma. Mushroom, Black Mushroom
The main reason is that there is no factory production, so it cannot be guaranteed to be picked at the best time of growth, and there is no way to develop a good way to preserve fresh products after picking
[0009] 4. The current cultivation method of cultivating false mushrooms with wrinkled caps generally has a slender stalk and a thin and soft cover. Because there is no strain selection, there is no selection of a variety with a thick stalk and a thick cover. poor

Method used

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  • Amauroderma rugosum strain and cultivation, picking and preservation method thereof
  • Amauroderma rugosum strain and cultivation, picking and preservation method thereof
  • Amauroderma rugosum strain and cultivation, picking and preservation method thereof

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Embodiment 1

[0055] The cultivation, collection, drying method of embodiment 1 new wrinkled cap pseudo-sedum strain RFC

[0056] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed;

[0057] The formula of the mother seed medium is: 20% of potato, 1.5% of agar, 1% of glucose, 0.2% of fish meal, 0.1% of potassium dihydrogen phosphate, and the balance is water.

[0058] S2 original seed preparation: the original seed culture material with a moisture content of 63% and a pH value of 5.4-5.6 was put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother species into a sterilized bag containing the original seed compost, and cultivate it at 22-28°C until the hyphae are covered with the fungus bag; the original seed...

Embodiment 2

[0064] Cultivation, collection, drying method of embodiment 2 new wrinkled cap false mushroom classification RFC

[0065] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed; mother seed medium formula It is: 25% of potato, 1.3% of agar, 1.5% of glucose, 0.2% of fish meal, 0.1% of potassium dihydrogen phosphate, and the balance is water.

[0066] S2 Preparation of the original seed: The original seed culture material with a pH value of 5.4-5.6 is put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother species into a sterilized bag containing the original seed culture material, and cultivate it at 22-28°C until the hyphae are covered with the bacteria bag; the original seed culture material incl...

Embodiment 3

[0071] Cultivation, collection, drying method of embodiment 3 new wrinkled cap false mushroom classification RFC

[0072] S1 Mother seed preparation: use the mother seed culture medium to prepare the test tube slope, insert the new Pseudomonas rugosa strain RFC into the test tube slope, and cultivate the inoculated test tube at a constant temperature of 22-28°C to obtain the mother seed; mother seed medium formula For: potato 22%, agar 1.4%, glucose 1.7%, fish meal 0.2%, potassium dihydrogen phosphate 0.1%, the balance is water

[0073] S2 Preparation of the original seed: The original seed culture material with a pH value of 5.4-5.6 is put into a bacteria bag and sealed, and then autoclaved at 108°C-125°C for 3 hours. Under aseptic conditions, insert the mother seed into the sterilization bag with the original seed culture material, and cultivate it at 22-28°C until the mycelium is covered with the bacteria bag; the original seed culture material includes 40% bamboo chips, fi...

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Abstract

The invention provides an Amauroderma rugosum strain and a cultivation method thereof. The 18s sequence of the Amauroderma rugosum strain is a nucleotide sequence as shown in SEQ ID NO.1, the 28S sequence of the Amauroderma rugosum strain is a nucleotide sequence as shown in SEQ ID NO.2, and the ITS sequence of the Amauroderma rugosum strain is a nucleotide sequence as shown in SEQ ID NO.3. According to the Amauroderma rugosum strain, Amauroderma rugosum stems are thick and strong, the diameters of the Amauroderma rugosum stems are 0.5-1.2 cm, the lengths of the stems are 7-30 cm, the Amauroderma rugosum stems are thick and hard, the diameters of the Amauroderma rugosum stems can reach 3-15 cm, the thicknesses of the Amauroderma rugosum stems are 0.5-1.0 cm, mature parts are dark, tender parts are snow white, the Amauroderma rugosum strain is rich in fragrance and superior in appearance and taste, the Amauroderma rugosum strain can survive in a culture material without Chinese Taiwan acacia basswood components, hypha growth speed is high, and the Amauroderma rugosum strain is the optimal strain at present.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a new strain of Pseudomonas rugosa and its cultivation, picking and preservation methods. Background technique [0002] Amauroderma rugosum, also known as Amauroderma rugosum, also known as black zhi, black zhi, black zhi, belongs to the phylum Fungi, the class Lamimycetes, the order Aphromycetes, the family Ganodermaceae, and the genus Amauroderma. Because the fruiting body will secrete blood-like red juice when touched by hard objects, it is also called blood ganoderma and fragrant blood ganoderma. The cap of the fruiting body of Falsemania chinensis is kidney-shaped, semicircular, with radiating deep wrinkles and fine hairs, often with concentric rings, thin edges, and later thickens to become flat and wavy (Zhao Jiding, Zhang Xiaoqing "Chinese Fungus Journal" "Volume 18), is a rare medicinal fungus unique to tropical and subtropical regions (Zhao Jiding, 1992; Bi Zhishu, 1994). It h...

Claims

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Application Information

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IPC IPC(8): A01G18/40A01G18/00A01G18/20A01G18/70A23L3/40A23L3/36
CPCY02W30/40
Inventor 陈丽丽李治君陈靖思张煜隆罗林华相杰
Owner 福州美利莎生物科技有限公司
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