Preparation method and application of natural radish blue pigment

A radish blue, natural technology, applied in the field of preparation of natural radish blue pigment, can solve the problems of easy degradation, complicated substances, low content of blue pigment, etc., and achieve the effect of increasing added value and good scientific research value

Active Publication Date: 2021-04-16
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The blue pigment content in radish is low, easy to degrade, affected by many factors, sensitive to light, heat, acid and alkali, and the substances in the radish system are complicated, so it is not easy to purify and prepare natural blue pigment

Method used

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  • Preparation method and application of natural radish blue pigment
  • Preparation method and application of natural radish blue pigment
  • Preparation method and application of natural radish blue pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the preparation of radish blue pigment

[0043] (1) Preparation of blue pigment reactant

[0044] Prepare 0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 0.29mol / L hydrogen peroxide solution, 30unit / mL, 300unit / mL tyrosinase (PPO), 30unit / mL , 300unit / mL peroxidase solution (POD); according to figure 1 Add the solution sequentially, respectively 1: substrate: 1mL 0.5mg / mL 4-oxindole-3-methylglucosinolate solution; 2: substrate + H 2 o 2 : 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution and 14uL0.29mol / L hydrogen peroxide solution; 3: Substrate + POD: 1mL0.5mg / mL 4-hydroxy Indole-3-methylglucosinolate solution and 100uL300unit / mL peroxidase solution; 4: substrate+H 2 o 2 +POD: 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 14uL0.29mol / L hydrogen peroxide solution and 100uL300unit / mL peroxidase solution; 5: Substrate+ h 2 o 2 +High POD: 1mL0.5mg / mL 4-oxindole-3-methylglucosinolate solution, 14uL0.29mol / L hydrogen peroxide solution a...

Embodiment 2

[0056] Embodiment 2, the antioxidant activity of radish blue pigment

[0057] (1) Determination of the oxygen radical absorption capacity (Oxygen radical absorbance capacity, ORAC) of purified blue pigment

[0058] Solution preparation:

[0059] 1) Phosphate buffer solution (PBS): Accurately measure an appropriate amount of phosphoric acid and dilute it with high-purity water to obtain a 75mmol / L phosphoric acid solution; weigh 8.56g of dipotassium hydrogen phosphate and dissolve it in 500mL of high-purity water, adjust the pH to 7.4 with the phosphoric acid solution to obtain 75mmol / L Phosphate buffer at pH 7.4;

[0060] 2) Sodium fluorescein (FL): 22.5mg fluorescein sodium salt was dissolved in 50ml PBS; then 50μL was dissolved in 10mL PBS;

[0061] 3) 2,2'-amidinopropane hydrochloride (AAPH): 162mg dissolved in 5mL PBS;

[0062] 4) Preparation of blue pigment: take 1 mg of blue pigment after freeze-drying and prepare 5, 10, 20, 40, 80 mg / mL blue pigment solutions with wa...

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Abstract

The invention discloses a preparation method and application of natural radish blue pigment. The preparation method comprises the following steps: S1, under the catalysis of peroxidase, reacting 4-hydroxyindole-3-methyl glucosinolate with hydrogen peroxide to obtain a blue pigment reactant; and S2, separating and purifying the blue pigment reactant by adopting HPLC (High Performance Liquid Chromatography), collecting a first absorption peak with the detection wavelength of 630nm, and concentrating and freeze-drying to obtain the blue pigment. The natural radish blue pigment prepared by the method has oxygen free radical absorption capacity and capacity of scavenging DPPH free radicals and ABTS free radicals, so that the natural radish blue pigment has oxidation resistance. The radish blue pigment is purified and prepared by adopting a simulation system, not only has market potential, but also is the premise of property and function research of a single natural radish blue pigment, and has good scientific research value. The natural radish blue pigment obtained by adopting the in-vitro portable preparation method can provide possibility for subsequent property research and application of the natural radish blue pigment.

Description

technical field [0001] The invention relates to a preparation method and application of natural radish blue pigment, belonging to the field of agricultural product processing. Background technique [0002] Radish has high nutritional value and is rich in vitamin C, folic acid, minerals, polyphenols and glucosinolates. It is a root vegetable of cruciferous plants; radish has anti-inflammatory and antioxidant activities, and prevents cancer. Economically important vegetable; not only widely grown in China, but also one of the most popular vegetables in the world. Specific varieties of radishes can turn blue when stored under certain conditions for 3 to 6 days after harvesting. This blue substance is a naturally formed pigment substance. Natural pigments are mainly red and yellow, and blue pigment is very rare. The absorption wavelength range is 580-760nm. "Word expresses. In addition, as a natural pigment, it is safe and non-toxic, and has great application potential in foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C09B61/00
Inventor 王丹赵晓燕马越张亚倩王宇滨
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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